Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
Arch Biochem Biophys. 2012 May;521(1-2):71-6. doi: 10.1016/j.abb.2012.03.013. Epub 2012 Mar 20.
To investigate the absorption and metabolism of 4-hydroxyderricin and xanthoangelol, we established an analytical method based on liquid chromatography-tandem mass spectrometry and measured these compounds in the plasma, urine, feces, liver, kidney, spleen, muscle and white adipose tissues of mice orally administered with Ashitaba extract (50-500mg/kg body weight). 4-Hydroxyderricin and xanthoangelol were quickly absorbed into the plasma, with time-to-maximum plasma concentrations of 2 and 0.5h for 4-hydroxyderricin and xanthoangelol, respectively. Although these compounds have similar structures, the total plasma concentration of 4-hydroxyderricin and its metabolites was approximately 4-fold greater than that of xanthoangelol and its metabolites at 24h. 4-Hydroxyderricin and xanthoangelol were mostly excreted in their aglycone forms and related metabolites (glucuronate and/or sulfate forms) in urine between 2 and 4h after oral administration of Ashitaba extract. On the other hand, these compounds were only excreted in their aglycone forms in feces. When tissue distribution of 4-hydroxyderricin and xanthoangelol was estimated 2h after administration of Ashitaba extract, both compounds were detected in all of the tissues assessed, mainly in their aglycone forms, except in the mesenteric adipose tissue. These results suggest that 4-hydroxyderricin and xanthoangelol are rapidly absorbed and distributed to various tissues.
为了研究 4-羟基当归素和黄当归醇的吸收和代谢情况，我们建立了一种基于液相色谱-串联质谱的分析方法，并测量了口服 Ashitaba 提取物（50-500mg/kg 体重）的小鼠血浆、尿液、粪便、肝、肾、脾、肌肉和白色脂肪组织中这些化合物的含量。4-羟基当归素和黄当归醇迅速被吸收到血浆中，达到最大血浆浓度的时间分别为 2 和 0.5 小时。尽管这两种化合物具有相似的结构，但在 24 小时时，4-羟基当归素及其代谢物的总血浆浓度约为黄当归醇及其代谢物的 4 倍。4-羟基当归素和黄当归醇主要以苷元形式及其代谢物（葡萄糖醛酸和/或硫酸盐形式）在口服 Ashitaba 提取物后 2-4 小时内从尿液中排出。另一方面，这些化合物仅以苷元形式从粪便中排出。当在给予 Ashitaba 提取物 2 小时后估计 4-羟基当归素和黄当归醇的组织分布时，两种化合物均存在于所有评估的组织中，主要以苷元形式存在，肠系膜脂肪组织除外。这些结果表明，4-羟基当归素和黄当归醇被迅速吸收并分布到各种组织中。
