Son Dong Ju, Park Ye Oak, Yu Chengguang, Lee Sung Eun, Park Young Hyun
a School of Applied Bioscience, Kyungpook National University , Daegu 702-701 , South Korea.
Nat Prod Res. 2014;28(24):2312-6. doi: 10.1080/14786419.2014.931389. Epub 2014 Jul 14.
Platelet aggregation is fundamental to a wide range of physiological and pathological processes, including the induction of thrombosis and arteriosclerosis. Anti-platelet activity of a crude methanol extract and solvent fractions of Ashitaba roots (Angelica keiskei Koidz.) was evaluated using a turbidimetric method using washed rabbit platelets. We identified the anti-platelet activities of two chalcones, 4-hydroxyderricin and xanthoangelol, isolated from the ethyl acetate-soluble fraction of Ashitaba roots by using a bioassay-guided isolation method. 4-Hydroxyderricin and xanthoangelol effectively inhibited platelet aggregation induced by collagen (IC50 of 41.9 and 35.9 μM, respectively), platelet-activating factor (IC50 of 46.1 and 42.3 μM, respectively) and phorbol 12-myristate 13-acetate (IC50 of 16.5 and 45.9 μM, respectively). These compounds did not inhibit thrombin-induced platelet aggregation (IC50 of>80 μM). The results suggest that the chalcones 4-hydroxyderricin and xanthoangelol may be potent anti-thrombotic components of A. keiskei Koidz.
血小板聚集是广泛的生理和病理过程的基础，包括血栓形成和动脉硬化的诱导。使用洗涤过的兔血小板，通过比浊法评估明日叶根（当归明日叶）粗甲醇提取物和溶剂馏分的抗血小板活性。我们采用生物测定引导的分离方法，从明日叶根的乙酸乙酯可溶部分中鉴定出两种查耳酮——4-羟基德里辛和黄当归醇的抗血小板活性。4-羟基德里辛和黄当归醇分别有效抑制胶原蛋白诱导的血小板聚集（IC50分别为41.9和35.9 μM）、血小板活化因子诱导的血小板聚集（IC50分别为46.1和42.3 μM）以及佛波醇12-肉豆蔻酸酯13-乙酸酯诱导的血小板聚集（IC50分别为16.5和45.9 μM）。这些化合物不抑制凝血酶诱导的血小板聚集（IC50>80 μM）。结果表明，查耳酮4-羟基德里辛和黄当归醇可能是明日叶的有效抗血栓成分。
