Li Yongjia, Goto Tsuyoshi, Yamakuni Kanae, Takahashi Haruya, Takahashi Nobuyuki, Jheng Huei-Fen, Nomura Wataru, Taniguchi Masahiko, Baba Kimiye, Murakami Shigeru, Kawada Teruo
Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611-0011, Japan.
Research Unit for Physiological Chemistry, The Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Kyoto, Japan.
Lipids. 2016 Jul;51(7):787-95. doi: 10.1007/s11745-016-4154-9. Epub 2016 Apr 20.
Adipocyte differentiation plays a pivotal role in maintaining the production of small-size adipocytes with insulin sensitivity, and impaired adipogenesis is implicated in insulin resistance. 4-Hydroxyderricin (4-HD), a phytochemical component of Angelica keiskei, possesses diverse biological properties such as anti-inflammatory, antidiabetic, and antitumor. In the present study, we investigated the effects of 4-HD on adipocyte differentiation. 4-HD promoted lipid accumulation in 3T3-L1 cells, upregulated both peroxisome proliferator-activated receptor (PPAR)-γ mRNA and protein expression, and acted as a ligand for PPARγ in the luciferase assay. Moreover, 4-HD increased the mRNA and protein expression levels of adiponectin. Additionally, it promoted insulin-dependent glucose uptake into 3T3-L1 adipocytes and increased Akt phosphorylation and glucose transporter (GLUT) 4 mRNA expression. In summary, these findings suggest that 4-HD, which promoted adipogenesis and insulin sensitivity in 3T3-L1 cells, might be a phytochemical with potent insulin-sensitizing effects.
脂肪细胞分化在维持具有胰岛素敏感性的小尺寸脂肪细胞的产生中起关键作用，而成脂作用受损与胰岛素抵抗有关。4-羟基德里辛（4-HD）是明日叶的一种植物化学成分，具有多种生物学特性，如抗炎、抗糖尿病和抗肿瘤。在本研究中，我们研究了4-HD对脂肪细胞分化的影响。4-HD促进了3T3-L1细胞中的脂质积累，上调了过氧化物酶体增殖物激活受体（PPAR）-γ mRNA和蛋白表达，并在荧光素酶测定中作为PPARγ的配体发挥作用。此外，4-HD增加了脂联素的mRNA和蛋白表达水平。此外，它促进了胰岛素依赖的葡萄糖摄取到3T3-L1脂肪细胞中，并增加了Akt磷酸化和葡萄糖转运蛋白（GLUT）4 mRNA表达。总之，这些发现表明，在3T3-L1细胞中促进脂肪生成和胰岛素敏感性的4-HD可能是一种具有强大胰岛素增敏作用的植物化学物质。
