Skuce P J, Johnston C F, Fairweather I, Halton D W, Shaw C, Buchanan K D
Department of Medicine, Queen's University of Belfast, Northern Ireland.
Cell Tissue Res. 1990 Sep;261(3):573-81. doi: 10.1007/BF00313537.
The presence and distribution of neuropeptides belonging to the pancreatic polypeptide family have been demonstrated by an indirect immunofluorescence technique in the nervous systems of adult male and female Schistosoma mansoni. Seven antisera of differing regional specificity to pancreatic polypeptide (PP), peptide YY (PYY) and neuropeptide Y (NPY) were employed on both whole-mount and cryostat-sectioned material. Positive immunoreactivity (IR) was obtained with all antisera except an N-terminally-directed antiserum to NPY. In the CNS, immunoreactivity was restricted to cell bodies and nerve fibres in the anterior ganglia, central commissure and dorsal and ventral nerve cords of both sexes, whereas, in the PNS, positive-IR was present in the plexuses innervating the subtegumental musculature and the oral and ventral suckers. Intense immunoreactivity was observed in a plexus of nerve fibres and cell bodies in the lining of the gynaecophoric canal and in fine nerve fibres innervating the dorsal tubercles of the male. In contrast, in the female, strong immunoreactivity was evident in nerve plexuses innervating the lining of the ovovitelline duct and in the wall of the ootype, but most notably in a cluster of cells in the region of Mehlis' gland. Results suggest that molecules with C-terminal homology to the PP-family are present in S. mansoni. These peptides would appear to be important regulatory molecules in the parasite's nervous system and may play a role in the control of egg production.
利用间接免疫荧光技术，已在成年雄性和雌性曼氏血吸虫的神经系统中证实了属于胰多肽家族的神经肽的存在和分布。对胰多肽（PP）、肽YY（PYY）和神经肽Y（NPY）具有不同区域特异性的七种抗血清被用于整装标本和低温恒温器切片材料。除了一种针对NPY的N端定向抗血清外，所有抗血清均获得了阳性免疫反应（IR）。在中枢神经系统中，免疫反应仅限于两性的前神经节、中央连合以及背侧和腹侧神经索中的细胞体和神经纤维，而在周围神经系统中，阳性IR存在于支配皮下肌肉组织以及口吸盘和腹吸盘的神经丛中。在抱雌沟内衬的神经纤维和细胞体丛以及支配雄性背结节的细神经纤维中观察到强烈的免疫反应。相比之下，在雌性中，在支配卵黄管内衬的神经丛和卵模壁中，尤其是在梅氏腺区域的一群细胞中，明显存在强烈的免疫反应。结果表明，曼氏血吸虫中存在与PP家族具有C端同源性的分子。这些肽似乎是寄生虫神经系统中的重要调节分子，可能在控制产卵中发挥作用。
