• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过益生元纳米颗粒联合调节肠道微生物群和酶触发的结肠给药可改善小鼠结肠炎治疗。

Combining Gut Microbiota Modulation and Enzymatic-Triggered Colonic Delivery by Prebiotic Nanoparticles Improves Mouse Colitis Therapy.

作者信息

Li Hui, Cheng Yu, Cui Luwen, Yang Zizhen, Wang Jingyi, Zhang Zixuan, Chen Kaiwei, Zhao Cheng, He Ningning, Li Shangyong

机构信息

School of Basic Medicine, Qingdao Medical College, Qingdao University, Qingdao, China.

Department of Abdominal Ultrasound, The Affiliated Hospital of Qingdao University, Qingdao, China.

出版信息

Biomater Res. 2024 Aug 13;28:0062. doi: 10.34133/bmr.0062. eCollection 2024.

DOI:10.34133/bmr.0062
PMID:39140035
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11321063/
Abstract

The efficacy of ulcerative colitis (UC) therapy is closely connected to the composition of gut microbiota in the gastrointestinal tract. Prebiotic-based nanoparticles (NPs) provide a more precise approach to alleviate UC via modulating gut microbiota dysbiosis. The present study develops an efficient prebiotic-based colon-targeted drug delivery system (PCDDS) by using prebiotic pectin (Pcn) and chitosan (Csn) polysaccharides as a prebiotic shell, with the anti-inflammatory drug sulfasalazine (SAS) loaded into a poly(lactic-co-glycolic acid) (PLGA) core to construct SAS@PLGA-Csn-Pcn NPs. Then, we examine its characterization, cellular uptake, and in vivo therapeutic efficacy. The results of our study indicate that the Pcn/Csn shell confers efficient pH-sensitivity properties. The gut microbiota-secreted pectinase serves as the trigger agent for Pcn/Csn shell degradation, and the resulting Pcn oligosaccharides possess a substantial prebiotic property. Meanwhile, the formed PCDDSs exhibit robust biodistribution and accumulation in the colon tissue, rapid cellular uptake, efficient in vivo therapeutic efficacy, and modulation of gut microbiota dysbiosis in a mouse colitis model. Collectively, our synthetic PCDDSs demonstrate a promising and synergistic strategy for UC therapy.

摘要

溃疡性结肠炎（UC）治疗的疗效与胃肠道中肠道微生物群的组成密切相关。基于益生元的纳米颗粒（NPs）通过调节肠道微生物群失调，为缓解UC提供了一种更精确的方法。本研究通过使用益生元果胶（Pcn）和壳聚糖（Csn）多糖作为益生元外壳，开发了一种高效的基于益生元的结肠靶向给药系统（PCDDS），将抗炎药物柳氮磺胺吡啶（SAS）负载到聚乳酸-乙醇酸共聚物（PLGA）核中，构建了SAS@PLGA-Csn-Pcn NPs。然后，我们研究了其特性、细胞摄取和体内治疗效果。我们的研究结果表明，Pcn/Csn外壳具有高效的pH敏感性。肠道微生物群分泌的果胶酶作为Pcn/Csn外壳降解的触发剂，产生的Pcn寡糖具有显著的益生元特性。同时，形成的PCDDSs在结肠组织中表现出强大的生物分布和积累、快速的细胞摄取、高效的体内治疗效果以及对小鼠结肠炎模型中肠道微生物群失调的调节作用。总的来说，我们合成的PCDDSs为UC治疗展示了一种有前景的协同策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/473cab5eecfc/bmr.0062.fig.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/a60b09f79be7/bmr.0062.fig.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/4bad3ad9a422/bmr.0062.fig.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/5f70fab3af34/bmr.0062.fig.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/155bd762086e/bmr.0062.fig.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/1922616c3378/bmr.0062.fig.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/a4eb3ac25e96/bmr.0062.fig.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/473cab5eecfc/bmr.0062.fig.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/a60b09f79be7/bmr.0062.fig.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/4bad3ad9a422/bmr.0062.fig.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/5f70fab3af34/bmr.0062.fig.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/155bd762086e/bmr.0062.fig.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/1922616c3378/bmr.0062.fig.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/a4eb3ac25e96/bmr.0062.fig.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da66/11321063/473cab5eecfc/bmr.0062.fig.007.jpg

相似文献

1
Combining Gut Microbiota Modulation and Enzymatic-Triggered Colonic Delivery by Prebiotic Nanoparticles Improves Mouse Colitis Therapy.通过益生元纳米颗粒联合调节肠道微生物群和酶触发的结肠给药可改善小鼠结肠炎治疗。
Biomater Res. 2024 Aug 13;28:0062. doi: 10.34133/bmr.0062. eCollection 2024.
2
An efficient enzyme-triggered controlled release system for colon-targeted oral delivery to combat dextran sodium sulfate (DSS)-induced colitis in mice.一种高效的酶触发控制释放系统，用于结肠靶向口服递药，以治疗小鼠葡聚糖硫酸钠（DSS）诱导的结肠炎。
Drug Deliv. 2021 Dec;28(1):1120-1131. doi: 10.1080/10717544.2021.1934189.
3
Targeted modulation of intestinal epithelial regeneration and immune response in ulcerative colitis using dual-targeting bilirubin nanoparticles.采用双靶向胆红素纳米粒靶向调控溃疡性结肠炎的肠道上皮细胞再生和免疫反应。
Theranostics. 2024 Jan 1;14(2):528-546. doi: 10.7150/thno.87739. eCollection 2024.
4
Preventive and Prebiotic Effect of α-Galacto-Oligosaccharide against Dextran Sodium Sulfate-Induced Colitis and Gut Microbiota Dysbiosis in Mice.α-半乳糖寡糖对葡聚糖硫酸钠诱导的小鼠结肠炎和肠道微生物失调的预防和益生菌作用。
J Agric Food Chem. 2021 Aug 25;69(33):9597-9607. doi: 10.1021/acs.jafc.1c03792. Epub 2021 Aug 11.
5
Bacillus coagulans in Combination with Chitooligosaccharides Regulates Gut Microbiota and Ameliorates the DSS-Induced Colitis in Mice.凝结芽孢杆菌联合壳寡糖通过调节肠道菌群改善 DSS 诱导的小鼠结肠炎。
Microbiol Spectr. 2022 Aug 31;10(4):e0064122. doi: 10.1128/spectrum.00641-22. Epub 2022 Jul 28.
6
Gut microbiota mediates the anti-colitis effects of polysaccharides derived from Kishinouye in mice.肠道微生物群介导来自漆姑草的多糖对小鼠的抗结肠炎作用。
Food Funct. 2023 Feb 21;14(4):1989-2007. doi: 10.1039/d2fo02712g.
7
Gut microbiota-based discovery of Houttuyniae Herba as a novel prebiotic of Bacteroides thetaiotaomicron with anti-colitis activity.基于肠道微生物组的研究发现鱼腥草是具抗结肠炎活性的拟杆菌属双歧杆菌的新型益生元。
Biomed Pharmacother. 2024 Mar;172:116302. doi: 10.1016/j.biopha.2024.116302. Epub 2024 Feb 21.
8
Berberine Depresses Inflammation and Adjusts Smooth Muscle to Ameliorate Ulcerative Colitis of Cats by Regulating Gut Microbiota.小檗碱通过调节肠道微生物群来抑制炎症和调节平滑肌，从而改善猫的溃疡性结肠炎。
Microbiol Spectr. 2022 Dec 21;10(6):e0320722. doi: 10.1128/spectrum.03207-22. Epub 2022 Oct 26.
9
Gegen Qinlian decoction ameliorates TNBS-induced ulcerative colitis by regulating Th2/Th1 and Tregs/Th17 cells balance, inhibiting NLRP3 inflammasome activation and reshaping gut microbiota.葛根芩连汤通过调节 Th2/Th1 和 Tregs/Th17 细胞平衡、抑制 NLRP3 炎性小体激活和重塑肠道微生物群来改善 TNBS 诱导的溃疡性结肠炎。
J Ethnopharmacol. 2024 Jun 28;328:117956. doi: 10.1016/j.jep.2024.117956. Epub 2024 Feb 29.
10
Colon-targeted delivery of cyclosporine A using dual-functional Eudragit FS30D/PLGA nanoparticles ameliorates murine experimental colitis.利用双功能 Eudragit FS30D/PLGA 纳米粒实现环孢素 A 的结肠靶向递释，改善了实验性结肠炎小鼠的病情。
Int J Nanomedicine. 2018 Feb 28;13:1225-1240. doi: 10.2147/IJN.S157566. eCollection 2018.

引用本文的文献

1
Synbiotic yogurt with nanoparticle entrapped rice straw hemicellulose for immediate probiotic support and prebiotic delivery.含有纳米颗粒包裹稻草半纤维素的合生元酸奶，用于即时益生菌支持和益生元递送。
NPJ Sci Food. 2025 Aug 18;9(1):177. doi: 10.1038/s41538-025-00539-z.
2
Nanomedicine Strategies in the Management of Inflammatory Bowel Disease and Colorectal Cancer.纳米医学在炎症性肠病和结直肠癌治疗中的策略
Int J Mol Sci. 2025 Jul 4;26(13):6465. doi: 10.3390/ijms26136465.
3
Preparation and property evaluation of oral colon targeted protein delivery system with sodium alginate and chitosan.

本文引用的文献

1
Recent progress on engineered micro/nanomaterials mediated modulation of gut microbiota for treating inflammatory bowel disease.工程化微/纳材料介导的调控肠道微生物群治疗炎症性肠病的最新进展。
J Control Release. 2024 Jun;370:43-65. doi: 10.1016/j.jconrel.2024.04.014. Epub 2024 Apr 17.
2
Poly(D,l-lactide-co-glycolide) particles are metabolised by the gut microbiome and elevate short chain fatty acids.聚（D，L-丙交酯-共-乙交酯）颗粒由肠道微生物群代谢并提高短链脂肪酸水平。
J Control Release. 2024 May;369:163-178. doi: 10.1016/j.jconrel.2024.03.039. Epub 2024 Mar 26.
3
Targeted modulation of intestinal epithelial regeneration and immune response in ulcerative colitis using dual-targeting bilirubin nanoparticles.
海藻酸钠和壳聚糖口服结肠靶向蛋白质递送系统的制备与性能评价
Sci Rep. 2025 Jul 1;15(1):21598. doi: 10.1038/s41598-025-04983-5.
4
Plant polysaccharide-capped nanoparticles: A sustainable approach to modulate gut microbiota and advance functional food applications.植物多糖包覆的纳米颗粒：一种调节肠道微生物群并推进功能性食品应用的可持续方法。
Compr Rev Food Sci Food Saf. 2025 Mar;24(2):e70156. doi: 10.1111/1541-4337.70156.
5
Recent development of micro-nano carriers for oral antineoplastic drug delivery.用于口服抗肿瘤药物递送的微纳米载体的最新进展。
Mater Today Bio. 2025 Jan 3;30:101445. doi: 10.1016/j.mtbio.2025.101445. eCollection 2025 Feb.
6
The microbiome's influence on obesity: mechanisms and therapeutic potential.微生物群对肥胖的影响：机制与治疗潜力。
Sci China Life Sci. 2025 Mar;68(3):657-672. doi: 10.1007/s11427-024-2759-3. Epub 2024 Nov 28.
采用双靶向胆红素纳米粒靶向调控溃疡性结肠炎的肠道上皮细胞再生和免疫反应。
Theranostics. 2024 Jan 1;14(2):528-546. doi: 10.7150/thno.87739. eCollection 2024.
4
Engineered extracellular vesicles: A new approach for targeted therapy of tumors and overcoming drug resistance.工程化细胞外囊泡：肿瘤靶向治疗及克服耐药性的新方法。
Cancer Commun (Lond). 2024 Feb;44(2):205-225. doi: 10.1002/cac2.12518. Epub 2023 Dec 28.
5
Ulcerative Colitis in Adults: A Review.成人溃疡性结肠炎：综述。
JAMA. 2023 Sep 12;330(10):951-965. doi: 10.1001/jama.2023.15389.
6
Author Correction: Chronic exposure to synthetic food colorant Allura Red AC promotes susceptibility to experimental colitis via intestinal serotonin in mice.作者更正：长期暴露于合成食用色素诱惑红AC通过肠道5-羟色胺增加小鼠对实验性结肠炎的易感性。
Nat Commun. 2023 May 30;14(1):3125. doi: 10.1038/s41467-023-38903-w.
7
Rosmarinic Acid Restores Colonic Mucus Secretion in Colitis Mice by Regulating Gut Microbiota-Derived Metabolites and the Activation of Inflammasomes.迷迭香酸通过调节肠道微生物群衍生的代谢产物和炎性小体的激活来恢复结肠炎小鼠的结肠黏液分泌。
J Agric Food Chem. 2023 Mar 22;71(11):4571-4585. doi: 10.1021/acs.jafc.2c08444. Epub 2023 Mar 7.
8
Application of Vibrational Spectroscopic Techniques in the Study of the Natural Polysaccharides and Their Cross-Linking Process.振动光谱技术在天然多糖及其交联过程研究中的应用。
Int J Mol Sci. 2023 Jan 30;24(3):2630. doi: 10.3390/ijms24032630.
9
Ameliorative effect of buckwheat polysaccharides on colitis via regulation of the gut microbiota.苦荞多糖通过调节肠道菌群对结肠炎的改善作用。
Int J Biol Macromol. 2023 Feb 1;227:872-883. doi: 10.1016/j.ijbiomac.2022.12.155. Epub 2022 Dec 20.
10
Rationally Designed Oral DOX Gels for Colon-Specific Administration.用于结肠特异性给药的合理设计口服阿霉素凝胶剂
Gels. 2022 Nov 22;8(12):759. doi: 10.3390/gels8120759.