Lutter Erika I, Martens Craig, Hackstadt Ted
Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
Comp Funct Genomics. 2012;2012:362104. doi: 10.1155/2012/362104. Epub 2012 Feb 21.
Chlamydia spp. are obligate intracellular pathogens that replicate within a vacuole termed the inclusion. Chlamydiae extensively modify the inclusion membrane via the insertion of chlamydial inclusion membrane proteins (Incs) which decorate the cytosolic face of the inclusion. We have assessed the overall relatedness and phylogeny of Incs in order to identify potential evolutionary trends. Despite a high degree of conservation among Incs within C. trachomatis serovars, phylogenetic analysis showed that some Incs cluster according to clinical groupings suggesting that certain Incs may contribute to tissue tropism. Bioinformatic predictions identified Incs in five chlamydial species: 55 in C. trachomatis, 68 in C. felis, 92 in C. pneumoniae, 79 in C. caviae, and 54 in C. muridarum. Inc homologues were compared between chlamydial species and 23 core Incs were identified as shared among all species. Genomic expansion of Incs was identified in C. pneumoniae, C. caviae, and C. felis but not C. trachomatis or C. muridarum.
衣原体属是专性细胞内病原体，在一个称为包涵体的液泡内进行复制。衣原体通过插入装饰包涵体胞质面的衣原体包涵体膜蛋白（Incs），对包涵体膜进行广泛修饰。我们评估了Incs的整体相关性和系统发育，以确定潜在的进化趋势。尽管沙眼衣原体血清型内的Incs之间具有高度保守性，但系统发育分析表明，一些Incs根据临床分组聚类，这表明某些Incs可能与组织嗜性有关。生物信息学预测在五种衣原体物种中鉴定出Incs：沙眼衣原体中有55种，猫衣原体中有68种，肺炎衣原体中有92种，豚鼠衣原体中有79种，鼠衣原体中有54种。对衣原体物种之间的Inc同源物进行了比较，并鉴定出23种核心Incs为所有物种所共有。在肺炎衣原体、豚鼠衣原体和猫衣原体中发现了Incs的基因组扩张，但在沙眼衣原体或鼠衣原体中未发现。
