Skenderidis Prodromos, Lampakis Dimitrios, Giavasis Ioannis, Leontopoulos Stefanos, Petrotos Konstantinos, Hadjichristodoulou Christos, Tsakalof Andreas
Department of Medicine, Lab of Hygiene and Epidemiology, University of Thessaly, Papakyriazi 22, 41222 Larissa, Greece.
Department of Biosystems Engineering/Agricultural Technology, Technological Educational Institute of Thessaly, 41110 Larissa, Greece.
Antioxidants (Basel). 2019 Mar 10;8(3):60. doi: 10.3390/antiox8030060.
In this study, the content composition and antioxidant activity of goji berry fruits from two species ( and ) were assessed. The total carbohydrate and phenolic contents were evaluated using attenuated total reflection Fourier-transform infrared (ATR-FT-IR) spectroscopy, while the antioxidant activity of fruits was examined with two in vitro methods, which are based on the scavenging activity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethyl-benzthiazoline-sulfonic acid) (ABTS•⁺) free radicals. The fatty-acid profile was determined using gas chromatography coupled with mass spectrometry (GC-MS). The results of this study indicate that the fruits of present higher concentrations in carbohydrates and phenolics than Mill. fruits. Furthermore, the antioxidant activity based on the half maximal inhibitory concentration (IC) measurements of DPPH• and ABTS•⁺ free-radical scavenging was higher in than Mill. Also, the GCMS analysis confirms the high levels of linoleic, palmitic, and oleic acids contained in the fruits of both species. Finally, the results of this study clearly show that the concentration of bioactive and antioxidant molecules is higher in than in fruits, which was also confirmed by ATR-FT-IR measurements.
在本研究中，对两种枸杞果实的成分组成和抗氧化活性进行了评估。使用衰减全反射傅里叶变换红外光谱（ATR-FT-IR）对总碳水化合物和酚类含量进行了评估，同时采用两种基于2,2-二苯基-1-苦基肼（DPPH•）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS•⁺）自由基清除活性的体外方法检测了果实的抗氧化活性。使用气相色谱-质谱联用（GC-MS）测定脂肪酸谱。本研究结果表明，[第一种枸杞]果实中的碳水化合物和酚类物质浓度高于宁夏枸杞（Lycium barbarum L.）果实。此外，基于DPPH•和ABTS•⁺自由基清除的半数抑制浓度（IC）测量的抗氧化活性，[第一种枸杞]高于宁夏枸杞（Lycium barbarum L.）。同样，GC-MS分析证实两种枸杞果实中均含有高水平的亚油酸、棕榈酸和油酸。最后，本研究结果清楚地表明，[第一种枸杞]果实中生物活性和抗氧化分子的浓度高于宁夏枸杞（Lycium barbarum L.）果实，这也得到了ATR-FT-IR测量的证实。
