白细胞介素-1与多囊卵巢综合征肥胖之间的相关性

[Correlation between interleukin-1 and the obesity of polycystic ovary syndrome].

作者信息

Yang Yan, Qiao Jie, Li Mei-zhi

机构信息

Center of Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2012 Jan;47(1):9-13.

PMID:22455686

Abstract

OBJECTIVE

To investigate the correlation between interleukin-1β (IL-1β), interleukin-1 receptor antagonist (IL-1ra) and the obesity of polycystic ovary syndrome (PCOS).

METHODS

(1) From Oct. 2006 to Jan. 2007, 118 PCOS patients were enrolled in this study in Peking University Third Hospital, which were divided into 56 patients in obese PCOS group and 62 patients in non-obese PCOS group according to the WHO International Obesity Task Force Asia-Pacific criteria [body mass index (BMI) 25 kg/m(2)]. The polymorphism of IL-1β gene promoter region, exon-5 and intron 2 of IL-1ra gene were detected by PCR. (2) Twenty-nine obese PCOS patients and 31 non-obese PCOS patients were selected randomizedly serum levels of IL-1β, IL-1ra were measured by ELISA, in the mean time, serum levels of fasting glucose, fasting insulin and the total white blood cell, hypersensitive C-reactive protein levels were measured.

RESULTS

(1) Genetic test:the frequency of TT genotype and T allele of IL-1β promoter region (-511) in obese PCOS patients were significantly higher than those in non-obese patients (44.6% vs. 11.3%, 63.4% vs. 39.5%, all P < 0.05). The frequency of IL-1raI/V genotype and V allele of IL-1ra gene were 19.6% and 9.8% in obese PCOS patients, which were significantly higher than those in non-obese group (3.2% and 1.6%, P < 0.05). (2) Serological test:serum level of IL-1β and IL-1ra of (149 ± 36) and (284 ± 97) ng/L in obese PCOS group which were significantly higher than those in non-obese PCOS group [(96 ± 42) and (208 ± 84) ng/L, P < 0.05]. Fasting blood glucose, fasting insulin and hypersensitive C-reactive protein and white blood cell count were (5.1 ± 0.7) mmol/L, (17 ± 9) mU/L, (1.5 ± 0.6) mg/L and (7.0 ± 2.3) × 10(9)/L in obese PCOS group, which were significantly higher than in non-obese PCOS group [(4.9 ± 0.5) mmol/L, (11 ± 8) mU/L, (0.9 ± 0.4) mg/L and (5.9 ± 1.3) × 10(9)/L, P < 0.05]. (3)The correlation between interleukin and BMI: serum levels of IL-1β (r = 0.673) and IL-1ra (r = 0.557) were positively correlated with BMI in PCOS patients (P < 0.05).

CONCLUSIONS

Inflammatory factors IL-1β and IL-1ra had correlation with obesity of PCOS patients, PCOS patients who carried T allele of IL-1β gene promoter region (-511) and V allele of IL-1ra gene were high risk of obesity.

摘要

目的

探讨白细胞介素-1β（IL-1β）、白细胞介素-1受体拮抗剂（IL-1ra）与多囊卵巢综合征（PCOS）肥胖之间的相关性。

方法

（1）2006年10月至2007年1月，北京大学第三医院招募了118例PCOS患者，根据世界卫生组织国际肥胖特别工作组亚太地区标准[体重指数（BMI）25kg/m²]分为肥胖PCOS组56例和非肥胖PCOS组62例。采用聚合酶链反应（PCR）检测IL-1β基因启动子区、IL-1ra基因外显子5和内含子2的多态性。（2）随机选取29例肥胖PCOS患者和31例非肥胖PCOS患者，采用酶联免疫吸附测定法（ELISA）检测血清IL-1β、IL-1ra水平，同时检测空腹血糖、空腹胰岛素及白细胞总数、超敏C反应蛋白水平。

结果

（1）基因检测：肥胖PCOS患者IL-1β启动子区（-511）TT基因型和T等位基因频率显著高于非肥胖患者（44.6%对11.3%，63.4%对39.5%，均P<0.05）。肥胖PCOS患者IL-1ra基因I/V基因型和V等位基因频率分别为19.6%和9.8%，显著高于非肥胖组（3.2%和1.6%，P<0.05）。（2）血清学检测：肥胖PCOS组血清IL-1β和IL-1ra水平分别为（149±36）和（284±97）ng/L，显著高于非肥胖PCOS组[（96±42）和（208±84）ng/L，P<0.05]。肥胖PCOS组空腹血糖、空腹胰岛素、超敏C反应蛋白及白细胞计数分别为（5.1±0.7）mmol/L、（17±9）mU/L、（1.5±0.6）mg/L和（7.0±2.3）×10⁹/L，显著高于非肥胖PCOS组[（4.9±0.5）mmol/L、（11±8）mU/L、（0.9±0.4）mg/L和（5.9±1.3）×10⁹/L，P<0.05]。（3）白细胞介素与BMI的相关性：PCOS患者血清IL-1β（r=0.673）和IL-1ra（r=0.557）水平与BMI呈正相关（P<0.05）。