Ames Laboratory, U.S. Department of Energy, and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
Anal Chem. 2012 May 1;84(9):4111-7. doi: 10.1021/ac300249d. Epub 2012 Apr 10.
We introduce a precise three-dimensional (3D) localization method of spherical gold nanoparticle probes using model-based correlation coefficient mapping. To accomplish this, a stack of sample images at different z-positions are acquired, and a 3D intensity profile of the probe serving as the model is used to map out the positions of nanoparticles in the sample. By using this model-based correlation imaging method, precise localization can be achieved in imaging techniques with complicated point spread functions (PSF) such as differential interference contrast (DIC) microscopy. We demonstrated the localization precision of 4-7 nm laterally and 16 nm axially for 40-nm gold nanospheres at an imaging rate of 10 frames per second. The 3D superlocalization method was applied to tracking gold nanospheres during live endocytosis events.
我们介绍了一种使用基于模型的相关系数映射来实现球形金纳米粒子探针精确三维（3D）定位的方法。为此，我们获取了一组在不同 z 位置的样本图像，并使用作为模型的探针的 3D 强度分布来确定样本中纳米粒子的位置。通过使用这种基于模型的相关成像方法，可以在具有复杂点扩散函数（PSF）的成像技术中实现精确的定位，例如微分干涉对比（DIC）显微镜。我们以每秒 10 帧的速度展示了 40nm 金纳米球在侧向 4-7nm 和轴向 16nm 的定位精度。该 3D 超定位方法应用于跟踪活细胞内吞过程中的金纳米球。
