Ames Laboratory-United States Department of Energy and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
Anal Chem. 2010 Aug 1;82(15):6675-9. doi: 10.1021/ac101336d.
The wavelength dependence of plasmonic nanoparticles' contrasts in differential interference contrast (DIC) microscopy has been exploited previously for unambiguous identification and dynamic tracking of these nanoprobes in complex environments (Anal. Chem. 2009, 81, 9203-9208). In the present study, the suitability of multiplexing detection in DIC microscopy was investigated systematically with 19 kinds of nanoparticles of different materials and/or sizes. A unique DIC contrast spectrum was found for each kind of nanoparticle. Multiplexing detection was accomplished by measuring DIC contrasts at a minimum of two specific illumination wavelengths. The main advantages of DIC microscopy for multiplexing detection over other nonfluorescence techniques, such as dark field microscopy and surface-enhanced Raman scattering, were demonstrated by differentiating four kinds of nanoparticles on the cell membrane while providing high-contrast images of both the nanoprobes and cell features.
先前已经利用等离子体纳米粒子在微分干涉差（DIC）显微镜下的波长依赖性对比度来明确识别和动态跟踪这些纳米探针在复杂环境中的情况（分析化学。2009 年，81，9203-9208）。在本研究中，系统地研究了 19 种不同材料和/或尺寸的纳米粒子在 DIC 显微镜中的多重检测适用性。发现每种纳米粒子都具有独特的 DIC 对比度谱。通过在至少两个特定照明波长下测量 DIC 对比度来实现多重检测。通过在细胞膜上区分四种纳米粒子，同时提供高对比度的纳米探针和细胞特征图像，证明了 DIC 显微镜在多重检测方面优于其他非荧光技术（如暗场显微镜和表面增强拉曼散射）的主要优势。
