Gerasimova E N, Torkhovskaia T I, Perova N V, Bazanova E A
Vopr Med Khim. 1979 Jul-Aug;25(4):471-80.
Content of cholesterol and of collagen fractions (salt-, acid soluble and insoluble) in thoracal and peritoneal parts of aorta as well as concentration of products of collagen metabolism (protein-bound and free hydroxyproline) in blood plasma were studied in rabbits with various degree of atherosclerosis--separate lipid spots in aorta thoracal part (I group), total impairment of aorta (II group) and in control animals. Concentration of cholesterol and triglycerides was also studied in blood plasma and content of cholesterol, cholesterol and triglyceride esters, phospholipids and protein was measured in separate types of lipoproteins--lipoprotein of low (LPLD), of very low (LPVLD) and of high density (LPHD). Increase in content of cholesterol, salt-soluble and insoluble collagen from the both parts of aorta as well as in concentration of protein-bound and free hydroxyproline was found in rabbits of the II group as compared with the control animals. In animals of the I group content of collagen fractions in aorta was similar to that of control animals. Hypercholesterolemia, observed in both groups of rabbits, was most distinct in the II group, being related to the higher amount of cholesterol in LPLD fraction. The ratio of cholesterol esters, free cholesterol, triglycerides and phospholipids was similarly altered in LPVLD fraction of the groups of animals, mainly due to an increase in content of cholesterol esters. But rabbits of the I group were distinct from the II group of animals in the ratio of these components in LPLD: relative content of protein was lower and of cholesterol esters--higher in LPLD of the II group than of the I group. The data suggest that LPLD contained high amount of lipoproteins of intermediate density and cholesterol deposited into vessel wall from LPLD and lipoproteins of intermediate density at the increased rate. In LPHD from blood plasma of rabbits of the II group the ratios cholesterol/protein, cholesterol esters/protein as well as the molar ratio free cholesterol/phospholipids were increased; these data suggest that LPHD was saturated with cholesterol and LPHD appeared to lose the ability to eliminate cholesterol from the vessel wall. Differences in the degree of atherosclerotic impairments, existing between the I and II groups of animals, were apparently related to the variations in composition and content of lipoproteins, which regulate the level of cholesterol in vessel wall, as well as to content of collagen, i. e. they were due to features of metabolism of lipoproteins in blood plasma and of collagen in the vascular wall.
研究了不同动脉粥样硬化程度的兔子（主动脉胸段有单独脂质斑的兔子为I组，主动脉完全受损的兔子为II组）以及对照动物的主动脉胸段和腹段中胆固醇和胶原蛋白各组分（盐溶性、酸溶性和不溶性）的含量，以及血浆中胶原蛋白代谢产物（蛋白结合羟脯氨酸和游离羟脯氨酸）的浓度。还研究了血浆中胆固醇和甘油三酯的浓度，并测定了不同类型脂蛋白（低密度脂蛋白LPLD、极低密度脂蛋白LPVLD和高密度脂蛋白LPHD）中胆固醇、胆固醇酯、甘油三酯酯、磷脂和蛋白质的含量。与对照动物相比，II组兔子主动脉两部分的胆固醇、盐溶性和不溶性胶原蛋白含量以及蛋白结合羟脯氨酸和游离羟脯氨酸的浓度均增加。I组动物主动脉中胶原蛋白各组分的含量与对照动物相似。两组兔子均出现高胆固醇血症，II组最为明显，这与LPLD组分中胆固醇含量较高有关。两组动物的LPVLD组分中胆固醇酯、游离胆固醇、甘油三酯和磷脂的比例变化相似，主要是由于胆固醇酯含量增加。但I组兔子与II组动物在LPLD中这些成分的比例上存在差异：II组LPLD中蛋白质的相对含量低于I组，而胆固醇酯的相对含量高于I组。数据表明，LPLD含有大量中等密度脂蛋白，且LPLD和中等密度脂蛋白中胆固醇以更快的速度沉积到血管壁中。II组兔子血浆LPHD中胆固醇/蛋白质、胆固醇酯/蛋白质以及游离胆固醇/磷脂的摩尔比增加；这些数据表明LPHD被胆固醇饱和，且LPHD似乎失去了从血管壁清除胆固醇的能力。I组和II组动物之间动脉粥样硬化损伤程度的差异显然与调节血管壁胆固醇水平的脂蛋白组成和含量变化以及胶原蛋白含量有关，即它们是由于血浆中脂蛋白和血管壁中胶原蛋白的代谢特征所致。
