School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China.
Int J Biol Macromol. 2012 Jun 1;50(5):1207-11. doi: 10.1016/j.ijbiomac.2012.03.005. Epub 2012 Mar 19.
A novel neutral heteropolysaccharide (DDP-1-D) was purified from hot water extracts of dried stem of Dendrobium densiflorum by DEAE-52 and Sephacryl S-200 High-Resolution Chromatography. The heteropolysaccharide had an average molecular weight about 9440 Da. It was composed mainly of glucose and mannose in the ratio of 3.01:1. Structural features of DDP-1-D were elucidated by a combination of chemical and instrumental techniques, including FT-IR, GC-MS, periodate oxidation-Smith degradation, (1)H and (13)C NMR spectroscopies (including COSY, TOCSY, HSQC, and HMBC spectra). The results indicated that DDP-1-D is a mannoglucan and has a backbone consisting of (1→4)-linked α-D-Glcp, (1→6)-linked α-D-Glcp, (1→2)-linked α-D-Manp and (1→4)-linked β-Manp. This is the first study to provide clear evidence for the structure of the polysaccharide in D. densiflorum.
一种新型中性杂多糖（DDP-1-D）从铁皮石斛干茎的热水提取物中通过 DEAE-52 和 Sephacryl S-200 高分辨层析法纯化得到。该杂多糖的平均分子量约为 9440 Da。它主要由葡萄糖和甘露糖组成，比例为 3.01:1。通过化学和仪器技术的组合，包括傅里叶变换红外光谱（FT-IR）、气相色谱-质谱联用（GC-MS）、高碘酸盐氧化-Smith 降解、（1）H 和（13）C 核磁共振光谱（包括 COSY、TOCSY、HSQC 和 HMBC 谱），阐明了 DDP-1-D 的结构特征。结果表明，DDP-1-D 是一种甘露聚糖，其主链由（1→4）-连接的α-D-Glcp、（1→6）-连接的α-D-Glcp、（1→2）-连接的α-D-Manp 和（1→4）-连接的β-Manp 组成。这是首次对铁皮石斛多糖结构进行的明确研究。
