Université d'Avignon et des Pays de Vaucluse, INRA, UMR408, F-84000 Avignon, France.
Bioresour Technol. 2012 Jun;114:457-65. doi: 10.1016/j.biortech.2012.02.096. Epub 2012 Mar 7.
In order to comply with criteria of green chemistry concepts and sustainability, a new procedure has been performed for solvent-free ultrasound-assisted extraction (UAE) to extract lipids from fresh Nannochloropsis oculata biomass. Through response surface methodology (RSM) parameters affecting the oil recovery were optimized. Optimum conditions for oil extraction were estimated as follows: (i) 1000 W ultrasonic power, (ii) 30 min extraction time and (iii) biomass dry weight content at 5%. Yields were calculated by the total fatty acids methyl esters amounts analyzed by GC-FID-MS. The maximum oil recovery was around 0.21%. This value was compared with the one obtained with the conventional extraction method (Bligh and Dyer). Furthermore, effect of temperature on the yield was also investigated. The overall results show an innovative and effective extraction method adapted for microalgae oil recovery, without using solvent and with an enable scaling up.
为了符合绿色化学概念和可持续性的标准，我们采用了一种新的无溶剂超声辅助提取（UAE）方法，从新鲜的眼点拟微绿球藻生物质中提取脂质。通过响应面法（RSM）优化了影响油脂回收的参数。最佳的油脂提取条件估计如下：（i）超声功率 1000 W，（ii）提取时间 30 分钟，（iii）生物质干重含量 5%。通过气相色谱-飞行时间质谱法（GC-FID-MS）分析总脂肪酸甲酯的量来计算产率。最大油脂回收率约为 0.21%。将该值与传统提取方法（Bligh 和 Dyer）获得的值进行了比较。此外，还研究了温度对产率的影响。总的来说，该结果表明这是一种创新且有效的提取方法，适用于微藻油的回收，无需使用溶剂，并且可以进行放大。
