INRA, UMR 1163 Biotechnologie des Champignons Filamenteux, 13288 Marseille, France.
Bioresour Technol. 2012 Jun;114:589-96. doi: 10.1016/j.biortech.2012.03.009. Epub 2012 Mar 10.
Fusarium verticillioides secretes enzymes (secretome), some of which might be potentially useful for saccharification of lignocellulosic biomass since supplementation of commercial cellulases from Trichoderma reesei with the F. verticillioides secretome improved the enzymatic release of glucose, xylose and arabinose from wheat straw by 24%, 88% and 68%, respectively. Determination of enzymatic activities revealed a broad range of hemicellulases and pectinases poorly represented in commercial cocktails. Proteomics approaches identified 57 proteins potentially involved in lignocellulose breakdown among a total of 166 secreted proteins. This analysis highlighted the presence of carbohydrate-active enzymes (CAZymes) targeting pectin (from glycoside hydrolase families GH5, GH27, GH28, GH43, GH51, GH54, GH62, GH88 and GH93, polysaccharide lyase family PL4 and carbohydrate esterase family CE8) and hemicelluloses (from glycoside hydrolase families GH3, GH10, GH11, GH30, GH39, GH43 and GH67). These data provide a first step towards the identification of candidates to supplement T. reesei enzyme preparations for lignocellulose hydrolysis.
镰刀菌（Fusarium verticillioides）分泌的酶（分泌组），其中一些可能对木质纤维素生物质的糖化有用，因为用 F. verticillioides 分泌组补充商业里氏木霉纤维素酶，分别提高了从麦草中葡萄糖、木糖和阿拉伯糖的酶解释放 24%、88%和 68%。酶活性测定显示，商业酶制剂中缺乏广泛的半纤维素酶和果胶酶。蛋白质组学方法鉴定了总共 166 种分泌蛋白中 57 种可能参与木质纤维素分解的蛋白。该分析突出了存在针对果胶（来自糖苷水解酶家族 GH5、GH27、GH28、GH43、GH51、GH54、GH62、GH88 和 GH93、多糖裂解酶家族 PL4 和碳水化合物酯酶家族 CE8）和半纤维素（来自糖苷水解酶家族 GH3、GH10、GH11、GH30、GH39、GH43 和 GH67）的碳水化合物活性酶（CAZymes）。这些数据为鉴定补充里氏木霉酶制剂用于木质纤维素水解的候选物提供了第一步。
