Department of Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Alessandria, Italy.
Department of Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Alessandria, Italy.
J Biotechnol. 2018 Mar 20;270:21-29. doi: 10.1016/j.jbiotec.2018.01.018. Epub 2018 Jan 31.
Lignocellulose degradation is a challenging step for value added products and biofuels production. Cellulomonas fimi secretes complex mixtures of carbohydrate active enzymes (CAZymes) which synergistically degrade cellulose and hemicelluloses. Their characterization may provide new insights for enzymatic cocktails implementation. Bioinformatic analysis highlighted 1127 secreted proteins, constituting the in silico secretome, graphically represented in a 2DE map. According to Blast2GO functional annotation, many of these are involved in carbohydrates metabolism. In vivo secretomes were obtained, growing C. fimi on glucose, CMC or wheat straw for 24 h. Zymography revealed degradative activity on carbohydrates and proteomic analysis identified some CAZymes, only in secretomes obtained with CMC and wheat straw. An interaction between cellobiohydrolases is proposed as a strategy adopted by soluble multimodular cellulases. Such approach can be crucial for a better characterization and industrial exploitation of the synergism among C. fimi enzymes.
木质纤维素的降解对于增值产品和生物燃料的生产来说是一个具有挑战性的步骤。纤维单胞菌属能够分泌复杂的碳水化合物活性酶（CAZymes）混合物，这些酶协同作用降解纤维素和半纤维素。对它们的特性进行分析可以为酶鸡尾酒的应用提供新的见解。生物信息学分析突出了 1127 种分泌蛋白，构成了计算机模拟的分泌组，并以 2DE 图谱的形式进行了图形表示。根据 Blast2GO 的功能注释，其中许多与碳水化合物代谢有关。通过在葡萄糖、CMC 或麦草上培养纤维单胞菌属 24 小时获得了体内分泌组。酶谱分析显示在碳水化合物上具有降解活性，蛋白质组学分析鉴定了一些 CAZymes，仅在 CMC 和麦草获得的分泌组中存在。提出了细胞二糖水解酶之间的相互作用，作为可溶性多功能纤维素酶采用的一种策略。这种方法对于更好地描述和开发纤维单胞菌属酶之间的协同作用至关重要。
