Department of Chemistry, Physical & Theoretical Chemistry Laboratory, Oxford University, South Parks Road, Oxford OX1 3QZ, UK.
Analyst. 2012 May 21;137(10):2285-96. doi: 10.1039/c2an35090d. Epub 2012 Mar 29.
The physiological importance of glutathione and glutathione disulfide is evident from their implications in an array of medical conditions including diabetes, Parkinson's disease and cancer. As such the need for simple, rapid and cheap assays to aid clinical diagnostics and treatment is clear. These requirements are, in principle at least, ideally suited to electrochemical detection. Accordingly a large array of voltammetric methods ultimately aimed at making cheap and most likely disposable electrodes have been reported. This critical review analyses the context in which physiological glutathione measurement can be undertaken electrochemically and compares it to current assay approaches, while also covering the current literature for glutathione disulfide detection. The various characteristics and limitations of the methodologies are compared and contrasted, with the analytical parameters (matrix, pH, limit of detection, etc.) tabulated to aid comparison.
谷胱甘肽及其二硫化物在一系列医学病症（包括糖尿病、帕金森病和癌症）中的作用表明其具有重要的生理学意义。因此，需要简单、快速和廉价的检测方法来辅助临床诊断和治疗。从原则上讲，这些要求非常适合电化学检测。因此，已经报道了大量最终旨在制造廉价且可能是一次性电极的伏安法方法。本综述批判性地分析了可以通过电化学方法进行生理谷胱甘肽测量的背景，并将其与当前的检测方法进行了比较，同时还涵盖了谷胱甘肽二硫化物检测的当前文献。比较和对比了各种方法的特点和局限性，并列出了分析参数（基质、pH 值、检测限等）以方便比较。
