Williams R W, Fu Z S, Hinze W L
Department of Chemistry, Wake Forest University, Winston-Salem, North Carolina 27109.
J Chromatogr Sci. 1990 Jun;28(6):292-302. doi: 10.1093/chromsci/28.6.292.
Aqueous solutions of bile salts, i.e. sodium cholate (NaC), sodium deoxycholate (NaDC), and sodium taurocholate (NaTC), are characterized and evaluated as reversed-phase liquid chromatographic (RPLC) mobile phases. The separation of the ASTM-recommended RPLC test mix in addition to more than 50 other compounds on a C18 column demonstrates the viability of these bile salts as HPLC mobile phases. The Armstrong-Nome theory was applied and found to adequately describe the partitioning behavior of solutes eluted with these bile salts at low surfactant concentrations. The effect of alcohol additives on chromatographic retention and efficiency was also assessed. Not only are the bile salt molecules rigid and chiral, but they form helical micellar aggregates as well. Consequently, many isomeric compounds can be easily resolved with this mobile phase additive. The base-line resolution of some binaphthyl-type enantiomers with a standard C18 column and the bile salt micellar mobile phases is also demonstrated. In addition, these bile salt mobile phases may be preferable to conventional hydroorganic mobile phase systems for the separation of many classes of routine compounds. A brief prospectus on the future utilization of bile salts in liquid chromatography is presented.
对胆盐的水溶液，即胆酸钠（NaC）、脱氧胆酸钠（NaDC）和牛磺胆酸钠（NaTC）进行了表征，并将其评估为反相液相色谱（RPLC）流动相。除了在C18柱上对ASTM推荐的RPLC测试混合物以及50多种其他化合物进行分离外，还证明了这些胆盐作为HPLC流动相的可行性。应用了阿姆斯特朗-诺姆理论，发现该理论能够充分描述在低表面活性剂浓度下用这些胆盐洗脱的溶质的分配行为。还评估了醇添加剂对色谱保留和效率的影响。胆盐分子不仅刚性且具有手性，而且还形成螺旋状胶束聚集体。因此，使用这种流动相添加剂可以轻松分离许多异构体化合物。还展示了使用标准C18柱和胆盐胶束流动相对一些联萘型对映体的基线分离。此外，对于许多类常规化合物的分离，这些胆盐流动相可能比传统的水有机流动相系统更具优势。本文还简要介绍了胆盐在液相色谱未来应用方面的前景。
