Center for Sleep & Circadian Neurobiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104-3403, USA.
Sleep. 2012 Apr 1;35(4):545-57. doi: 10.5665/sleep.1744.
To determine the effect of different genetic backgrounds on demographic and environmental interventions that affect sleep and evaluate variance of these measures; and to evaluate sleep and variance of sleep behaviors in 6 divergent laboratory strains of common origin.
Assessment of the effects of age, sex, mating status, food sources, and social experience using video analysis of sleep behavior in 2 different strains of Drosophila, white(1118ex) (w(1118ex)) and white Canton-S (w(CS10)). Sleep was also determined for 6 laboratory strains of Canton-S and 3 inbred lines. The variance of total sleep was determined for all groups and conditions.
The circadian periods and the effects of age upon sleep were the same between w(1118ex) and w(CS10) strains. However, the w(1118ex) and w(CS10) strains demonstrated genotype-dependent differences in the effects upon sleep of sex, mating status, social experience, and being on different foods. Variance of total sleep was found to differ in a genotype dependent manner for interventions between the w(1118ex) and w(CS10) strains. Six different laboratory Canton-S strains were found to have significantly different circadian periods (P < 0.001) and sleep phenotypes (P < 0.001). Three inbred lines showed reduced variance for sleep measurements.
One must control environmental conditions in a rigorously consistent manner to ensure that sleep data may be compared between experiments. Genetic background has a significant impact upon changes in sleep behavior and variance of behavior due to demographic factors and environmental interventions. This represents an opportunity to discover new genes that modify sleep/wake behavior.
确定不同遗传背景对影响睡眠的人口统计学和环境干预措施的影响，并评估这些措施的变化；评估 6 种具有共同起源的不同实验室品系的睡眠和睡眠行为变化。
使用两种不同的果蝇（白色（1118ex）（w(1118ex)）和白色坎顿-S（w(CS10)））的睡眠行为视频分析，评估年龄、性别、交配状态、食物来源和社会经验的影响。还确定了 6 种坎顿-S 实验室品系和 3 种近交系的睡眠。所有组和条件下均确定了总睡眠时间的变化。
w(1118ex)和 w(CS10) 品系的昼夜周期和年龄对睡眠的影响相同。然而，w(1118ex)和 w(CS10)品系在性别、交配状态、社会经验以及食用不同食物对睡眠的影响方面表现出基因型依赖性差异。发现 w(1118ex)和 w(CS10) 品系之间的干预措施总睡眠时间变化存在基因型依赖性差异。发现 6 种不同的实验室坎顿-S 品系具有显著不同的昼夜周期（P < 0.001）和睡眠表型（P < 0.001）。三种近交系的睡眠测量值变化方差减小。
必须以严格一致的方式控制环境条件，以确保可以在实验之间比较睡眠数据。遗传背景对由于人口统计学因素和环境干预导致的睡眠行为变化和行为变化的变化有重大影响。这为发现新的基因来改变睡眠/觉醒行为提供了机会。
