蛋白质组学发现胃癌的肿瘤标志物。

Discovery of tumor markers for gastric cancer by proteomics.

机构信息

Department of Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan ; Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan ; Department of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Institute of Nuclear Energy Research, Atomic Energy Council, Executive Yuan, Taiwan.

出版信息

PLoS One. 2014 Jan 3;9(1):e84158. doi: 10.1371/journal.pone.0084158. eCollection 2014.

Gastric cancer (GC) has a high rate of morbidity and mortality among various cancers worldwide. The development of noninvasive diagnostic methods or technologies for tracking the occurrence of GC is urgent, and searching reliable biomarkers is considered.This study intended to directly discover differential biomarkers from GC tissues by two-dimension-differential gel electrophoresis (2D-DIGE), and further validate protein expression by western blotting (WB) and immunohistochemistry (IHC).Pairs of GC tissues (gastric cancer tissues and the adjacent normal tissues) obtained from surgery was investigated for 2D-DIEG.Five proteins wereconfirmed by WB and IHC, including glucose-regulated protein 78 (GRP78), glutathione s-transferase pi (GSTpi), apolipoprotein AI (ApoAI), alpha-1 antitrypsin (A1AT) and gastrokine-1 (GKN-1). Among the results, GRP78, GSTpi and A1ATwere significantlyup-regulated and down-regulated respectively in gastric cancer patients. Moreover, GRP78 and ApoAI were correlated with A1AT for protein expressions.This study presumes these proteins could be candidates of reliable biomarkers for gastric cancer.

胃癌（GC）是全球各种癌症中发病率和死亡率较高的一种癌症。因此，迫切需要开发用于跟踪 GC 发生的非侵入性诊断方法或技术，并寻找可靠的生物标志物。本研究旨在通过二维差异凝胶电泳（2D-DIGE）直接从 GC 组织中发现差异生物标志物，并通过 Western blot（WB）和免疫组织化学（IHC）进一步验证蛋白表达。对手术获得的 GC 组织（胃癌组织和相邻正常组织）进行了 2D-DIEG 研究。通过 WB 和 IHC 验证了 5 种蛋白质，包括葡萄糖调节蛋白 78（GRP78）、谷胱甘肽 S-转移酶 pi（GSTpi）、载脂蛋白 AI（ApoAI）、α-1 抗胰蛋白酶（A1AT）和胃动素-1（GKN-1）。结果表明，胃癌患者的 GRP78、GSTpi 和 A1AT 分别显著上调和下调。此外，GRP78 和 ApoAI 的蛋白表达与 A1AT 相关。本研究假设这些蛋白质可能是胃癌可靠生物标志物的候选者。