Department of Biochemistry, Erciyes University Faculty of Medicine, Kayseri, Turkey.
Sleep Med. 2012 Apr;13(4):414-8. doi: 10.1016/j.sleep.2011.11.012.
We aimed to determine the importance of oxidative stress in the pathogenesis of restless legs syndrome (RLS) by quantification of advanced oxidation protein products and total thiol levels (as markers of oxidative protein damage), nitric oxide levels (as an antioxidant and endothelial function), and malondialdehyde levels (as a marker of lipid peroxidation) in patients with RLS.
A total of 22 patients with primary RLS were enrolled in the study and 20 age-and-gender-matched healthy subjects were enrolled as a control group. Serum nitric oxide, malondialdehyde, thiol levels, and plasma advanced oxidation protein products levels were determined by spectrophotometric methods.
Serum nitric oxide and thiol levels were lower in the patient group than in controls (p = 0.007 and p = 0.017, respectively). Plasma advanced oxidation protein products levels and serum malondialdehyde levels were found to be higher in patients with RLS than in controls (p = 0.017 and p = 0.008, respectively). Serum malondialdehyde level was found to be positively correlated with plasma advanced oxidation protein products levels (p = 0.039). Serum thiol level was found to be negatively correlated with plasma advanced oxidation protein products levels (p = 0.030).
Increased advanced oxidation protein products, malondialdehyde levels, and decreased thiol and nitric oxide levels, may suggest that patients with RLS are under oxidative stress. Although both lipid peroxidation and protein oxidation may have a role in atherosclerosis in RLS, those factors may be related to the pathogenesis of RLS.
通过定量检测氧化应激蛋白产物和总巯基水平（氧化蛋白损伤的标志物）、一氧化氮水平（抗氧化剂和内皮功能的标志物）以及丙二醛水平（脂质过氧化的标志物），确定氧化应激在不安腿综合征（RLS）发病机制中的重要性。
共纳入 22 例原发性 RLS 患者作为病例组，同时纳入 20 例年龄和性别匹配的健康者作为对照组。采用分光光度法测定血清一氧化氮、丙二醛、巯基水平和血浆氧化应激蛋白产物水平。
病例组血清一氧化氮和巯基水平均低于对照组（p = 0.007 和 p = 0.017）。RLS 患者血浆氧化应激蛋白产物水平和血清丙二醛水平高于对照组（p = 0.017 和 p = 0.008）。血清丙二醛水平与血浆氧化应激蛋白产物水平呈正相关（p = 0.039）。血清巯基水平与血浆氧化应激蛋白产物水平呈负相关（p = 0.030）。
升高的氧化应激蛋白产物、丙二醛水平，以及降低的巯基和一氧化氮水平，可能提示 RLS 患者处于氧化应激状态。尽管脂质过氧化和蛋白氧化可能都与 RLS 中的动脉粥样硬化有关，但这些因素可能与 RLS 的发病机制有关。
