Singh A V, Singh S V, Sohal J S, Singh P K
Veterinary Microbiology Laboratory, Animal Health Division, Central Institute Makhdoom, PO - Farah, District Mathura-281122 (UP), India.
J Commun Dis. 2010 Jun;42(2):91-100.
Present study aimed to genotype Mycobacterium avium subspecies paratuberculosis (MAP) recovered from suspected and Crohn' s disease patients. A total of 32 MAP and DNA (directly from clinical samples) recovered from human origin were genotyped using IS 1311 PCR-REA. Isolates were cultured from stool, biopsies and blood clots of Crohn's disease patients, and stool samples of suspected (animal attendants, lab workers etc). Of the 32 MAP isolates belonging to 28 human beings, majority (84.3%) were genotyped as 'Bison type', while 21.7% were of 'cattle' and none was 'sheep' genotype. Study first time reports distribution of 'Cattle' and 'Bison type' 'genotypes in suspected and Crohn's patients on pilot scale in India. 'Bison type' genotype was predominant in the surveyed human population.
本研究旨在对从疑似患者和克罗恩病患者中分离出的副结核分枝杆菌（MAP）进行基因分型。使用IS 1311 PCR-REA对总共32株从人类样本中直接提取的MAP及其DNA进行了基因分型。分离株取自克罗恩病患者的粪便、活检组织和血凝块，以及疑似患者（动物饲养员、实验室工作人员等）的粪便样本。在这32株来自28人的MAP分离株中，大多数（84.3%）被基因分型为“野牛型”，而21.7%为“牛型”，没有“羊型”基因型。该研究首次在印度小规模报告了疑似患者和克罗恩病患者中“牛型”和“野牛型”基因型的分布情况。在被调查的人群中，“野牛型”基因型占主导地位。
