Aneesh E M, Vijayan V A
Vector Biology Research Laboratory, Department of Studies in Zoology, University of Mysore, Manasagangothri, Mysore -06, Karnataka, India.
J Commun Dis. 2010 Sep;42(3):201-7.
Carbofuran is a systemic insecticide/nematicide extensively employed in modern agriculture and public health to combat various insect pests and vectors. The development of insecticide resistance due to natural selection pressure by Anopheline and Culicine mosquitoes has created serious repercussions in mosquito control and disease eradication campaigns. In this regard Culex quinquefasciatus, a widely dispersed domestic mosquito and the only vector of lymphatic filariasis in India, was selected for the present experiment. In order to assess the resistance development in this species, it was subjected to carbofuran bioassay by employing WHO method for 10 generations. The results of larval selection test up to F10 revealed a significant increase in tolerance development in every generation compared to the susceptible F1. The results revealed up to 16.225 times tolerance after 10 generations. The LC50 observed in F10 is 1.298 ppm compared to 0.08 ppm in the F1. The implications of the observations will be discussed.
克百威是一种内吸性杀虫剂/杀线虫剂，在现代农业和公共卫生领域被广泛用于防治各种害虫和病媒。由于按蚊和库蚊的自然选择压力导致的抗药性发展，给蚊虫控制和疾病根除行动带来了严重影响。在这方面，本实验选择了广泛分布的家蚊致倦库蚊，它是印度淋巴丝虫病的唯一病媒。为了评估该物种的抗药性发展情况，采用世界卫生组织的方法对其进行了10代的克百威生物测定。直到F10的幼虫选择试验结果显示，与敏感的F1代相比，每一代的耐受性都有显著增加。结果显示，10代后耐受性提高了16.225倍。F10代观察到的LC50为1.298 ppm，而F1代为0.08 ppm。将讨论这些观察结果的意义。
