Dombrowski James E, Martin Ruth C
USDA-ARS, National Forage Seed Production Research Center, 3450 SW Campus Way, Corvallis, OR, 97331-7102, USA.
BMC Res Notes. 2018 Jan 29;11(1):79. doi: 10.1186/s13104-017-3076-9.
Previously we have shown that mechanical wounding and volatiles released from cut grass, activated a 46 and 44 kDa mitogen-activated protein kinase (MAPK) in the model grass species Lolium temulentum (Lt). MAPKs play an important role as signal relays that connect incoming stress signals and stress responses. Since green leaf volatiles (GLV) are released during wounding, we wanted determine if specific compounds contained in the GLV mixture or if GLV generated from other plant species could activate these Lt MAPKs.
Our analysis found that just a 1-min exposure to GLV was enough to activate the Lt 46 kDa MAPK within 3 min and the 44 kDa MAPK within 15 min. This activation pattern showed similar kinetics to those observed after wounding, and the GLV and wound activated bands associated with these MAPKs displayed identical migration on sodium dodecyl sulfate polyacrylamide gels. Thirteen different commercially available plant volatiles (alcohols, aldehydes and ketones) were tested and all thirteen volatile compounds were able to activate these same Lt MAPKs. Furthermore, GLV derived from three other grass species as well as tomato, a dicot, were also shown to activate these MAPKs in Lt.
此前我们已表明，机械损伤以及割草释放的挥发物可激活模式草种毒麦（Lt）中的一种46 kDa和一种44 kDa的丝裂原活化蛋白激酶（MAPK）。MAPK作为连接传入应激信号和应激反应的信号中继发挥着重要作用。由于绿叶挥发物（GLV）在受伤时会释放，我们想确定GLV混合物中含有的特定化合物，或者其他植物物种产生的GLV是否能激活这些Lt MAPK。
我们的分析发现，仅1分钟的GLV暴露就足以在3分钟内激活Lt 46 kDa MAPK，并在15分钟内激活44 kDa MAPK。这种激活模式显示出与受伤后观察到的类似动力学，并且与这些MAPK相关的GLV和伤口激活条带在十二烷基硫酸钠聚丙烯酰胺凝胶上显示出相同的迁移。测试了13种不同的市售植物挥发物（醇类、醛类和酮类），所有13种挥发性化合物都能够激活相同的Lt MAPK。此外，源自其他三种草种以及双子叶植物番茄的GLV也显示能激活Lt中的这些MAPK。
