开发和鉴定南美野生西番莲（西番莲科）微卫星标记。
Development and characterization of microsatellite markers for the wild South American Passiflora cincinnata (Passifloraceae).
机构信息
Centro de Biologia Molecular e Engenharia Genética and Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
出版信息
Am J Bot. 2012 Apr;99(4):e170-2. doi: 10.3732/ajb.1100477. Epub 2012 Apr 2.
PREMISE OF THE STUDY
We developed the first set of microsatellite markers for the passion fruit, Passiflora cincinnata, to provide tools for further study of its genetic diversity and to support current conservation and genetic studies.
METHODS AND RESULTS
We used a microsatellite-enriched library approach to isolate and characterize 25 new molecular markers. Seven primer pairs successfully amplified polymorphic microsatellite regions, with an average of five alleles per locus. The mean values of expected and observed levels of heterozygosity were 0.516 and 0.525, respectively.
CONCLUSIONS
The microsatellite markers identified in this study may be valuable tools for population genetic studies, and this set of markers also may be useful in the design of a genetic conservation strategy, mating system, and hybridization studies.
研究前提
我们开发了第一批西番莲属（Passiflora cincinnata）微卫星标记，为进一步研究其遗传多样性提供工具，并支持当前的保护和遗传研究。
方法和结果
我们使用微卫星富集文库方法分离和鉴定了 25 个新的分子标记。7 对引物成功扩增出多态性微卫星区域，每个位点平均有 5 个等位基因。期望和观察到的杂合度平均值分别为 0.516 和 0.525。
结论
本研究中鉴定的微卫星标记可能是群体遗传研究的有用工具，这组标记也可能有助于设计遗传保护策略、交配系统和杂交研究。
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