UMR DIADE, IRD, 34394 Montpellier, France.
Am J Bot. 2012 Mar;99(3):e105-7. doi: 10.3732/ajb.1100423. Epub 2012 Feb 21.
We developed nuclear microsatellite primers to explore the genetic diversity, population genetic structure, and evolutionary history of the fonio (Digitaria exilis), an understudied cereal cultivated in West Africa.
We used a microsatellite-enriched library approach to isolate and characterize 38 nuclear primer pairs (31 di-, five tri-, and two tetranucleotide repeats), of which 21 were polymorphic and exhibited a clear pattern in 36 accessions from West Africa. The number of alleles per locus ranged from two to 22, with a mean of 4.71, and expected heterozygosity ranged from 0.03 to 0.93.
The developed set of 21 polymorphic SSR markers will provide tools for population and evolutionary genetics studies of the cultivated fonio.
我们开发了核微卫星引物，以探索在西非种植的研究较少的谷物——珍珠粟（Digitaria exilis）的遗传多样性、种群遗传结构和进化历史。
我们使用微卫星富集文库方法分离和鉴定了 38 对核引物（31 对二核苷酸重复、5 对三核苷酸重复和 2 对四核苷酸重复），其中 21 对多态性，在来自西非的 36 个样本中表现出明显的模式。每个位点的等位基因数范围从 2 到 22，平均值为 4.71，预期杂合度范围从 0.03 到 0.93。
开发的这 21 个多态性 SSR 标记将为栽培珍珠粟的群体和进化遗传学研究提供工具。
