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一些当地水稻品种(水稻)种子中的蛋白质和氨基酸。

Proteins and amino acids of some local varieties of rice seeds (Oryza Sativa L.).

作者信息

Hagop E G, Younis S A, Shahatha H A

机构信息

Scientific Research Council, Biological Research Centre, Jadiriya, Baghdad, Iraq.

出版信息

Plant Foods Hum Nutr. 1990 Oct;40(4):309-15. doi: 10.1007/BF02193855.

DOI:10.1007/BF02193855
PMID:2247436
Abstract

Proteins and amino acids in four local rice (Oryza Sativa L.) varieties were identified. Albumin and globulin were extracted from rice seeds, and the major promoters of these proteins were investigated by polyacrylamide gel electrophoresis to show their patterns. Amino acid composition of the rice seed were determined quantitatively and qualitatively, and classified according to their acidic, basic and uncharged polar groups. Essential amino acids for each variety were determined, and the hydrophobicity index value of Amber 33 was (0.6078), Mishkhab 1 (0.63372), Hybrid 2 (0.6523) and Hwazawi (0.7411).

摘要

对四个当地水稻(Oryza Sativa L.)品种中的蛋白质和氨基酸进行了鉴定。从水稻种子中提取白蛋白和球蛋白,并通过聚丙烯酰胺凝胶电泳研究这些蛋白质的主要启动子,以显示它们的模式。对水稻种子的氨基酸组成进行了定量和定性测定,并根据其酸性、碱性和不带电荷的极性基团进行分类。测定了每个品种的必需氨基酸,琥珀33的疏水性指数值为(0.6078),米什卡卜1为(0.63372),杂交2为(0.6523),哈扎维为(0.7411)。

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本文引用的文献

1
Electrophoretic characterization of rice varieties using single seed (salt soluble) proteins.利用单粒种子(盐溶性)蛋白质进行水稻品种的电泳分析。
Theor Appl Genet. 1984 Aug;68(5):415-9. doi: 10.1007/BF00254809.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.