Mawal Y R, Mawal M R, Ranjekar P K
Biosci Rep. 1987 Jan;7(1):1-9. doi: 10.1007/BF01122721.
Rice albumin from Oryza sativa (Var. Basmati 370) accounts for about 5% of the total seed proteins. A major fraction of rice albumin has been found to be a glycoprotein which is a monomer of 60 kd having iso-electric point 6.54. When rice albumin is digested with trypsin, it shows the presence of 24 peptides as against 28 peptides which were estimated from its amino acid composition. This indicates the presence of a few peptides which resemble each other in their charge and Rf values. Antibodies against Con A purified rice albumin were affinity purified and were used to quantitate the rice albumin levels during post-anthesis by RIA and ELISA. The latter experiments reveal that maximum albumin is present between 18 and 20 days post-anthesis.
来自水稻（品种：巴斯马蒂370）的水稻白蛋白约占种子总蛋白的5%。已发现水稻白蛋白的主要部分是一种糖蛋白，它是一种60kd的单体，等电点为6.54。当用胰蛋白酶消化水稻白蛋白时，它显示出存在24种肽，而根据其氨基酸组成估计有28种肽。这表明存在一些在电荷和Rf值上彼此相似的肽。针对伴刀豆球蛋白A纯化的水稻白蛋白的抗体经过亲和纯化，并用于通过放射免疫分析（RIA）和酶联免疫吸附测定（ELISA）定量花后水稻白蛋白水平。后一项实验表明，花后18至20天白蛋白含量最高。
