Department of Food Science Technology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
Mediators Inflamm. 2012;2012:829650. doi: 10.1155/2012/829650. Epub 2012 Feb 9.
In the present study, the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis, to stimulate osteoblast function and inhibit the release of bone-resorbing mediators was investigated in osteoblastic MC3T3-E1 cells.
Osteoblast function was measured by cell growth, alkaline phosphatase activity, collagen synthesis, and mineralization. Glutathione content was also measured in the cells. Bone-resorbing cytokines, receptor activator of nuclear factor-κB ligand (RANKL), TNF-α, and IL-6 were measured with an enzyme immunoassay system.
Magnolol caused a significant elevation of cell growth, alkaline phosphatase activity, collagen synthesis, mineralization, and glutathione content in the cells (P < 0.05). Skeletal turnover is orchestrated by a complex network of regulatory factors. Among cytokines, RANKL, TNF-α, and IL-6 were found to be key osteoclastogenetic molecules produced by osteoblasts. Magnolol significantly (P < 0.05) decreased the production of osteoclast differentiation inducing factors such as RANKL, TNF-α, and IL-6 in the presence of antimycin A, which inhibits mitochondrial electron transport and has been used as an ROS generator.
Magnolol might be a candidate as an agent for the prevention of bone disorders such as osteoporosis.
在本研究中，研究了从厚朴中分离出的羟化联苯化合物厚朴酚刺激成骨细胞功能和抑制破骨细胞释放骨吸收介质的能力，在成骨细胞 MC3T3-E1 细胞中进行了研究。
通过细胞生长、碱性磷酸酶活性、胶原合成和矿化来测量成骨细胞功能。还测量了细胞中的谷胱甘肽含量。采用酶免疫分析系统测定破骨细胞生成的骨吸收细胞因子核因子-κB 配体（RANKL）、TNF-α和 IL-6。
厚朴酚可显著提高细胞生长、碱性磷酸酶活性、胶原合成、矿化和细胞内谷胱甘肽含量（P < 0.05）。骨骼代谢是由一个复杂的调节因子网络来调控的。在细胞因子中，RANKL、TNF-α和 IL-6 被认为是成骨细胞产生的关键破骨细胞生成分子。厚朴酚可显著（P < 0.05）降低抗霉素 A 存在时破骨细胞分化诱导因子如 RANKL、TNF-α和 IL-6 的产生，抗霉素 A 抑制线粒体电子传递，可作为 ROS 生成剂。
厚朴酚可能是预防骨质疏松等骨骼疾病的候选药物。
