Institute of Clinical Pathology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
Evid Based Complement Alternat Med. 2012;2012:701927. doi: 10.1155/2012/701927. Epub 2012 Mar 13.
Introduction. Several studies demonstrated that anti-inflammatory remedies exhibit excellent anti-neoplastic properties. An extract of Pluchea odorata (Asteraceae), which is used for wound healing and against inflammatory conditions, was fractionated and properties correlating to anti-neoplastic and wound healing effects were separated. Methods. Up to six fractionation steps using silica gel, Sephadex columns, and distinct solvent systems were used, and eluted fractions were analysed by thin layer chromatography, apoptosis, and proliferation assays. The expression of oncogenes and proteins regulating cell migration was investigated by immunoblotting after treating HL60 cells with the most active fractions. Results. Sequential fractionations enriched anti-neoplastic activities which suppressed oncogene expression of JunB, c-Jun, c-Myc, and Stat3. Furthermore, a fraction (F4.6.3) inducing or keeping up expression of the mobility markers MYPT, ROCK1, and paxillin could be separated from another fraction (F4.3.7), which inhibited these markers. Conclusions. Wound healing builds up scar or specific tissue, and hence, compounds enhancing cell migration support this process. In contrast, successful anti-neoplastic therapy combats tumour progression, and thus, suppression of cell migration is mandatory.
简介。多项研究表明，抗炎药物具有出色的抗肿瘤特性。一种用于治疗伤口和炎症的 Pluchea odorata（菊科）提取物经过分离，分离出与抗肿瘤和伤口愈合作用相关的成分。方法。使用硅胶、葡聚糖柱和不同溶剂系统进行多达六个分级步骤，并用薄层色谱、细胞凋亡和增殖测定分析洗脱的馏分。用最活跃的馏分处理 HL60 细胞后，通过免疫印迹法研究致癌基因和调节细胞迁移的蛋白质的表达。结果。连续分级富集了抗肿瘤活性，抑制了 JunB、c-Jun、c-Myc 和 Stat3 的致癌基因表达。此外，可以将诱导或维持运动标志物 MYPT、ROCK1 和桩蛋白表达的一个馏分（F4.6.3）与抑制这些标志物的另一个馏分（F4.3.7）分离。结论。伤口愈合会形成疤痕或特定组织，因此，增强细胞迁移的化合物会支持这一过程。相比之下，成功的抗肿瘤治疗会对抗肿瘤的进展，因此，抑制细胞迁移是必要的。
