Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104, Republic of Korea.
Skin Biotechnology Center, Kyung Hee University, Yongin, 17104, Republic of Korea.
J Microbiol. 2018 Dec;56(12):910-916. doi: 10.1007/s12275-018-8307-9. Epub 2018 Nov 27.
Zizania latifolia is a perennial herb belonging to the family Gramineae that has been used as a health food in Asian countries. In this study, we investigated the antimicrobial effect of Z. latifolia, which increased human beta-defensin 2 (hBD2) expression in HaCaT cells. hBD2 expression was further increased in cells treated with Z. latifolia extracts and subsequently infected with Staphylococcus aureus. Inversely, S. aureus infection decreased after treatment. The induction of hBD2 in HaCaT cells was mediated by the Toll-like receptor 2 (TLR2) signaling pathway, including the activation of extracellular signal-regulated kinase (ERK) and activator protein 1 (AP-1). Further study using siRNA revealed that hBD2 played an important role in the inhibition of S. aureus infection in HaCaT cells. Our data suggest that Z. latifolia extracts can be used as an antimicrobial ingredient for skin treatment formulas.
菰米是禾本科植物，属于多年生草本植物，在亚洲国家被用作保健食品。本研究发现，菰米提取物可增加 HaCaT 细胞中人β防御素 2（hBD2）的表达，且在后续金黄色葡萄球菌感染时，hBD2 的表达进一步增加。相反，金黄色葡萄球菌感染后经处理减少。HaCaT 细胞中 hBD2 的诱导是通过 Toll 样受体 2（TLR2）信号通路介导的，包括细胞外信号调节激酶（ERK）和激活蛋白 1（AP-1）的激活。使用 siRNA 的进一步研究表明，hBD2 在抑制 HaCaT 细胞金黄色葡萄球菌感染中发挥重要作用。我们的数据表明，菰米提取物可用作皮肤处理配方的抗菌成分。
