Nazaruk Jolanta, Karna Ewa, Kalemba Danuta
Department of Pharmacognosy, Medical University of Białystok, Mickiewicza 2a Str., 15-089 Białystok, Poland.
Nat Prod Commun. 2012 Feb;7(2):269-72.
The composition of the essential oils of Cirsium palustre and C. rivulare and their antiproliferative activity against breast adenocarcinoma cells (MCF-7 and MDA-MBA-231) were investigated. The essential oils obtained by hydro-distillation from the roots (yield 0.2 and 0.1% v/w, respectively), leaves and inflorescences (yield below 0.01%), were analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The composition of the essential oils in the respective organs of each plant differed considerably. On the other hand, similarities were observed in the composition of root and leaf oils. In C. palustre and C. rivulare root oil, 50 and 39 constituents were identified, accounting for 95.3% and 92.4% of the total content. The main components were aplotaxene and its derivatives, representing 78.6% and 46.6% of the total content. In leaf oils of both species, 59 and 49 compounds, respectively, were identified, representing 67.4% and 78.3% of the total content. The major constituents were beta-damascenone (4.1% and 13.4%, respectively) and beta-ionone (6.7% and 5.3%, respectively). Short-chain saturated and unsaturated aliphatic alcohols and aldehydes constituted another important group of compounds (17.7% and 9.0%, respectively). The essential oils of the roots have moderate anti-proliferative activity, with IC50 values ranging from 110 to 140 microg/mL. These concentrations were below the level able to inhibit the proliferation of healthy cells.
对沼生蓟（Cirsium palustre）和溪边蓟（C. rivulare）精油的成分及其对乳腺腺癌细胞（MCF - 7和MDA - MBA - 231）的抗增殖活性进行了研究。通过水蒸馏法从根部（产率分别为0.2%和0.1% v/w）、叶片和花序（产率低于0.01%）获得的精油，采用气相色谱 - 质谱联用（GC - MS）进行分析。每种植物各器官中精油的成分差异很大。另一方面，在根和叶精油的成分中观察到了相似性。在沼生蓟和溪边蓟的根油中，分别鉴定出50种和39种成分，占总含量的95.3%和92.4%。主要成分是阿朴他烯及其衍生物，分别占总含量的78.6%和46.6%。在两种植物的叶油中，分别鉴定出59种和49种化合物，占总含量的67.4%和78.3%。主要成分是β - 大马酮（分别为4.1%和13.4%）和β - 紫罗兰酮（分别为6.7%和5.3%）。短链饱和和不饱和脂肪醇及醛构成了另一组重要化合物（分别为17.7%和9.0%）。根精油具有中等抗增殖活性，IC50值在110至140μg/mL之间。这些浓度低于能够抑制健康细胞增殖的水平。
