Xie Wen-Jie, Sun Ting, Yang Xiao-Rong, Ma Ming
Department of Urology, The First Hospital Affiliated to Nanchnag University, Nanchang, Jiangxi 330006, China.
Zhonghua Nan Ke Xue. 2012 Mar;18(3):204-7.
To investigate the expressions of brain natriuretic peptide (BNP) and natriuretic peptide receptor (NPR-A) in the cord dorsal horn ganglion (DRG) of rat models of chronic nonbacterial prostatitis (CNP) and the relation of BNP and NPR-A with CNP-induced chronic pain.
We established CNP models in 30 healthy clean SD rats using Freund's complete adjuvant, and included another 10 in a sham-operation group. The prostate tissues were subjected to HE staining, and the expressions of BNP and NPR-A in the L5-S2 DRGs were detected by real-time PCR.
Higher degree of inflammation was related to longer modeling time. At 3, 7 and 10 days, the expressions of BNP in the CNP models were 2.16 +/- 0.35, 1.61 +/- 0.21 and 1.32 +/- 0.36, and those of NPR-A were 2.75 +/- 0.06, 2.15 +/- 0.15 and 1.04 +/- 0.13, respectively, significantly higher at 3 and 7 days as compared with the sham-operation group (P<0.05), but with no statistically significant difference at 10 days.
BNP and NPR-A are expressed in the L5-S2 DRGs of SD rats and their expressions can be upregulated by CNP. BNP and NPR-A may be involved in the mechanisms of CNP-induced pain.
探讨慢性非细菌性前列腺炎（CNP）大鼠模型脊髓背根神经节（DRG）中脑钠肽（BNP）和利钠肽受体（NPR-A）的表达，以及BNP和NPR-A与CNP所致慢性疼痛的关系。
采用弗氏完全佐剂对30只健康清洁级SD大鼠建立CNP模型，另取10只作为假手术组。对前列腺组织进行HE染色，采用实时荧光定量PCR检测L5-S2 DRG中BNP和NPR-A的表达。
炎症程度越高，建模时间越长。在第3、7和10天，CNP模型中BNP的表达分别为2.16±0.35、1.61±0.21和1.32±0.36，NPR-A的表达分别为2.75±0.06、2.15±0.15和1.04±0.13，第3天和第7天显著高于假手术组（P<0.05），但第10天差异无统计学意义。
BNP和NPR-A在SD大鼠L5-S2 DRG中表达，且其表达可被CNP上调。BNP和NPR-A可能参与了CNP所致疼痛的机制。
