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脑钠肽在非细菌性前列腺炎大鼠脊髓中枢的调节作用。

Regulatory role of BNP in the spinal center of rats with nonbacterial prostatitis.

机构信息

Department of Urology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China.

Department of Health Management Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China.

出版信息

Technol Health Care. 2024;32(S1):351-359. doi: 10.3233/THC-248031.

DOI:10.3233/THC-248031
PMID:38759060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11307007/
Abstract

BACKGROUND

A growing body of evidence has shown that activating spinal cord glial cells (typically astrocytes and microglial cells) is closely related to hyperpathia and persistent pain.

OBJECTIVE

To investigate the expression of GFAP and CR3/CD11b in cornu dorsale medullae spinalis of rats with nonbacterial prostatitis, to explore the therapeutic efficacy and action mechanism of intrathecal injection of BNP alleviating chronic neuropathic pain.

METHODS

Eighteen male SPF SD rats were randomly divided into sham operation control group, nonbacterial prostatitis group (NBP) and intrathecal injection BNP group, the NBP model was established by intraprostatic injection of CFA, and the spinal cord of L6-S1 segment was extracted seven days after intrathecal injection of BNP; The expression of GFAP and CR3/CD11b in dorsal horn of spinal cord were detected by immunofluorescence and Western blot.

RESULTS

The cumulative optical density values of GFAP and CR3/CD11b immunofluorescence assay in the NBP group were higher than those in the sham operation group, with statistical significance (p⁢ï⁢¼⁢ 0.01); The expression of GFAP and CR3/CD11b in intrathecal injection BNP group were lower than those in NBP group, the differences were statistically significant (p⁢ï⁢¼⁢ 0.01). Western blot results showed that the expression of GFAP and CR3/CD11B in NBP group were higher than those in sham operation group, with statistical significance (p⁢ï⁢¼⁢ 0.05). The expression of GFAP and CR3/CD11B in intrathecal injection BNP group were lower than those in NBP group, the differences were statistically significant (p⁢ï⁢¼⁢ 0.05).

CONCLUSION

Intrathecal injection of BNP can down-regulate the expressions of GFAP and CR3/CD11b in L6-S1 spinal cord of NBP rat model and to further inhibit chronic pain caused by NBP.

摘要

背景

越来越多的证据表明，激活脊髓神经胶质细胞（通常是星形胶质细胞和小胶质细胞）与痛觉过敏和持续性疼痛密切相关。

目的

探讨非细菌性前列腺炎大鼠脊髓背角 GFAP 和 CR3/CD11b 的表达，探讨鞘内注射脑钠肽（BNP）缓解慢性神经病理性疼痛的疗效及作用机制。

方法

将 18 只雄性 SPF 级 SD 大鼠随机分为假手术对照组、非细菌性前列腺炎组（NBP 组）和鞘内注射 BNP 组，通过前列腺内注射 CFA 建立 NBP 模型，鞘内注射 BNP 后 7 天提取 L6-S1 段脊髓；免疫荧光和 Western blot 检测脊髓背角 GFAP 和 CR3/CD11b 的表达。

结果

NBP 组 GFAP 和 CR3/CD11b 免疫荧光检测的累积光密度值高于假手术组，差异有统计学意义（p⁢ï⁢¼⁢ 0.01）；鞘内注射 BNP 组 GFAP 和 CR3/CD11b 的表达低于 NBP 组，差异有统计学意义（p⁢ï⁢¼⁢ 0.01）。Western blot 结果显示，NBP 组 GFAP 和 CR3/CD11B 的表达均高于假手术组，差异有统计学意义（p⁢ï⁢¼⁢ 0.05）。鞘内注射 BNP 组 GFAP 和 CR3/CD11B 的表达均低于 NBP 组，差异有统计学意义（p⁢ï⁢¼⁢ 0.05）。

结论

鞘内注射 BNP 可下调 NBP 大鼠模型 L6-S1 脊髓 GFAP 和 CR3/CD11b 的表达，进一步抑制 NBP 引起的慢性疼痛。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/3766c3986642/thc-32-thc248031-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/ba149022671c/thc-32-thc248031-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/bf959acb8a15/thc-32-thc248031-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/d8a13f2c58e2/thc-32-thc248031-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/078a3cd68acd/thc-32-thc248031-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/e39f2d076057/thc-32-thc248031-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/3766c3986642/thc-32-thc248031-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/ba149022671c/thc-32-thc248031-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/bf959acb8a15/thc-32-thc248031-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/d8a13f2c58e2/thc-32-thc248031-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/078a3cd68acd/thc-32-thc248031-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/e39f2d076057/thc-32-thc248031-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67c/11307007/3766c3986642/thc-32-thc248031-g006.jpg

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Pain Res Manag. 2023 Nov 13;2023:2061632. doi: 10.1155/2023/2061632. eCollection 2023.
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Expression and Regulation of Brain Natriuretic Peptide and Natriuretic Peptide Receptor A (NPR-A) in L6-S1 Dorsal Root Ganglia in a Rat Model of Chronic Nonbacterial Prostatitis.脑钠肽和利钠肽受体 A（NPR-A）在慢性非细菌性前列腺炎大鼠 L6-S1 背根神经节中的表达和调节。
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