Hanfling Sarina N, Parikh Toral, Mayhew Allison, Robinson Erin, Graham James, Gomez-Lobo Veronica, Maher Jacqueline Y
Eunice Kennedy Shriver National Institute of Child Health and Human Development, Pediatric and Adolescent Gynecology Division, Bethesda, Maryland.
Children's National Hospital, Pediatric and Adolescent Gynecology Division, Department of Surgery, Northwest, Washington, D.C.
F S Rep. 2021 Apr 8;2(3):296-299. doi: 10.1016/j.xfre.2021.03.007. eCollection 2021 Sep.
To report two cases of mature oocytes found in prepubertal girls undergoing ovarian tissue cryopreservation (OTC).
Case report.
Large tertiary care children's hospital and a private fertility clinic.
An 8-year-old prepubertal girl with β-thalassemia and a 2-year-old girl with sickle cell disease who both underwent OTC before bone marrow transplantations.
Laparoscopic right oophorectomy was performed in each patient. The ovarian cortical tissue was processed for slow freezing and long-term storage, and all oocytes were subsequently vitrified.
Oocytes found at the time of OTC processing for fertility preservation.
After a complete right oophorectomy, one mature metaphase II oocyte was discovered on tissue processing for OTC in each patient. Neither patient has yet returned for use of tissue or oocytes.
To our knowledge, this is the first report of mature oocytes found during prepubertal OTC processing. These findings may indicate the need for increased research regarding prepubertal oocyte development and suggest that the technique of examining the media for both mature and immature oocytes at the time of OTC should become more widespread and perhaps recommended in prepubertal patients to optimize fertility preservation methods.
报告两例在接受卵巢组织冷冻保存（OTC）的青春期前女孩中发现成熟卵母细胞的病例。
病例报告。
大型三级儿童专科医院和一家私立生育诊所。
一名8岁患β地中海贫血的青春期前女孩和一名2岁患镰状细胞病的女孩，她们在进行骨髓移植前均接受了OTC。
对每位患者进行腹腔镜下右卵巢切除术。将卵巢皮质组织进行慢速冷冻和长期保存，随后对所有卵母细胞进行玻璃化冷冻。
在进行OTC以保存生育能力时发现的卵母细胞。
在完整切除右侧卵巢后，对每位患者进行OTC组织处理时均发现了一枚成熟的中期II卵母细胞。两名患者均尚未返回使用组织或卵母细胞。
据我们所知，这是关于青春期前OTC过程中发现成熟卵母细胞的首例报告。这些发现可能表明需要加强对青春期前卵母细胞发育的研究，并提示在OTC时检查培养液中成熟和未成熟卵母细胞的技术应得到更广泛应用，或许还应推荐用于青春期前患者以优化生育力保存方法。
