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土壤中分离得到的解淀粉芽孢杆菌对马拉硫磷的转化。

Transformation of malathion by Lysinibacillus sp. isolated from soil.

机构信息

Department of Biotechnology, Panjab University, Chandigarh, 160014, India.

出版信息

Biotechnol Lett. 2012 May;34(5):863-7. doi: 10.1007/s10529-011-0837-8. Epub 2012 Apr 3.

Abstract

An axenic bacterial strain, Lysinibacillus sp. KB1, was isolated from malathion-contaminated soil. It tolerated malathion up to 0.15 % and, under aerobic conditions, utilized it as sole carbon source. 20 % malathion and 47 % malaoxon were degraded out of the initially provided malathion. Two metabolites, mal-monocarboxylic acid and mal-dicarboxylic acid, were detected within 7 days at 30 °C. Esterase activity of the strain was 240 ± 2.5 U/ml after 7 days of growth. Sterilized soil mixed with malathion showed rapid degradation of malathion when inoculated with strain KB1 as compared to the uninoculated soil.

摘要

从马拉硫磷污染的土壤中分离到一株无菌细菌菌株,Lysinibacillus sp. KB1。它能耐受高达 0.15%的马拉硫磷,在好氧条件下,能将其作为唯一的碳源利用。初始提供的马拉硫磷中有 20%和 47%被降解为马拉氧磷和硫代磷酸。在 30°C 下,7 天内检测到两种代谢物,即单羧酸和二羧酸。经过 7 天的生长,该菌株的酯酶活性为 240±2.5 U/ml。与未接种的土壤相比,当用 KB1 菌株接种灭菌土壤并混合马拉硫磷时,马拉硫磷能迅速降解。

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