Pernas-Pleite Carlos, Conejo-Martínez Amparo M, Marín Irma, Abad José P
Department of Molecular Biology, Biology Building, Faculty of Sciences, Autonomous University of Madrid, Cantoblanco, 28049 Madrid, Spain.
Biomolecules. 2025 May 16;15(5):731. doi: 10.3390/biom15050731.
Antibiotic-resistant bacteria pose problems for infection prevention and treatment, so developing new procedures or substances against infection is mandatory. Silver nanomaterials are among the more promising antibacterial agents. Herein, we describe the biogenic synthesis of silver nanoparticles (AgNPs) using culture broths from an undescribed species of . Culture broths with or without NaCl and from the exponential and stationary growth phases produced four AgNP types. Nanoparticles' shapes were quasi-spherical, with core sizes of 7.5-14.7 nm and hydrodynamic diameters of 48.5-80.2 nm. All the AgNPs contained Ag crystals and some AgCl ones. Moreover, their coronas presented different proportions of carbohydrates, proteins, and aliphatic compounds. The AgNPs were good antibacterial agents against six bacterial species, three Gram-positive and three Gram-negative, with MICs of 0.3-9.0 µg/mL. Their activity was higher against the Gram-negative bacteria and particularly against . These AgNPs acted synergistically with several of the fifteen tested antibiotics. Interestingly, AgNP combinations with some of these inhibited the growth of antibiotic-resistant bacteria, as in the case of for streptomycin and for colistin. The ROS production by and when treated with most AgNPs suggested different mechanisms for bacterial killing depending on the AgNP.
抗生素耐药细菌给感染预防和治疗带来了问题，因此开发新的抗感染程序或物质势在必行。银纳米材料是比较有前景的抗菌剂之一。在此，我们描述了使用一种未描述物种的培养液生物合成银纳米颗粒（AgNP）的过程。添加或不添加氯化钠、处于指数生长期和稳定生长期的培养液产生了四种类型的AgNP。纳米颗粒的形状为准球形，核心尺寸为7.5 - 14.7纳米，流体动力学直径为48.5 - 80.2纳米。所有的AgNP都含有银晶体，还有一些氯化银晶体。此外，它们的冠层呈现出不同比例的碳水化合物、蛋白质和脂肪族化合物。这些AgNP对六种细菌具有良好的抗菌作用，其中三种革兰氏阳性菌和三种革兰氏阴性菌，最低抑菌浓度为0.3 - 9.0微克/毫升。它们对革兰氏阴性菌的活性更高，尤其是对……。这些AgNP与十五种测试抗生素中的几种具有协同作用。有趣的是，AgNP与其中一些抗生素的组合抑制了抗生素耐药细菌的生长，如链霉素的……和黏菌素的……。……和……在用大多数AgNP处理时产生的活性氧表明，根据AgNP的不同，细菌杀灭机制也不同。
