Lindhardt Pedersen T, Kjaersgaard E, Plesner T
Department of Medicine and Hematology C, Gentofte Hospital, Hellerup, Denmark.
Scand J Clin Lab Invest. 1990 Oct;50(6):657-61. doi: 10.3109/00365519009089184.
We have tested a Quantitative Buffy Coat (QBC) instrument (Becton Dickinson, USA), and compared results obtained with this instrument to results obtained with standard methods in a haematology clinic. The basic principle of the method is a classical haematocrit centrifuge. The analysis provides a haematocrit value, platelet count, a total white blood count, and separates the white blood cells in granulocytes and mononuclear cells (lymphocytes plus monocytes). The instrument is easy to use but requires a trained observer. All results are available in 15 min. We have found the accuracy of the method good for all parameters. The precision of the instrument is good but for estimation of granulocytes and lymphocytes plus monocytes we did not find the high sensitivity claimed by the manufacturer (manufacturer's lower limit 0.02 x 10(9)/l; standard deviation for low levels of granulocytes: 0.300 x 10(9)/l and lymphocytes plus monocytes: 0.235 x 10(9)/l). A large fraction of samples (leukocytes 27%, platelets 40%) from a haematological clinic falls beyond the limits for reliable results set by the manufacturer, which reduces the utility of the instrument in such patients. Furthermore, in 21% and 10% of samples within the recommended range for leukocytes and platelets, respectively, QBC results could not be read owing to ill-defined boundaries. For granulocytes and lymphocytes plus monocytes 25% and 34% of the samples, respectively, could not be read owing to ill-defined boundaries. The instrument is not constructed to protect against blood contamination of the centrifuge and, therefore, the safety of the instrument is not satisfactory.
我们测试了一台定量血沉棕黄层（QBC）仪器（美国贝克顿·迪金森公司），并将该仪器得出的结果与血液学诊所采用标准方法得出的结果进行了比较。该方法的基本原理是经典的血细胞比容离心机。该分析可提供血细胞比容值、血小板计数、白细胞总数，并将白细胞分离为粒细胞和单核细胞（淋巴细胞加单核细胞）。该仪器易于使用，但需要经过培训的操作人员。所有结果在15分钟内即可得出。我们发现该方法对所有参数的准确性都很好。该仪器的精密度良好，但对于粒细胞以及淋巴细胞加单核细胞的估计，我们并未发现制造商所宣称的高灵敏度（制造商规定的下限为0.02×10⁹/L；粒细胞低水平时的标准差为0.300×10⁹/L，淋巴细胞加单核细胞的标准差为0.235×10⁹/L）。血液学诊所的很大一部分样本（白细胞为27%，血小板为40%）超出了制造商设定的可靠结果范围，这降低了该仪器在此类患者中的实用性。此外，在白细胞和血小板分别处于推荐范围内的样本中，分别有21%和10%由于界限不明确而无法读取QBC结果。对于粒细胞和淋巴细胞加单核细胞的样本，分别有25%和34%由于界限不明确而无法读取。该仪器未设计防止离心机受到血液污染，因此，该仪器的安全性并不令人满意。
