Bron S, Luxen E, Venema G, Trautner T A
Mol Gen Genet. 1980;179(1):103-10. doi: 10.1007/BF00268451.
The effects of restriction in vivo by competent B. subtilis R cells and in vitro by purified endonuclease BsuR on transformation and transfection with native and denatured DNA were investigated. The results show that transformation by either native, or denatured DNA is not affected by restriction, whereas transfection both with native and denatured SPP1 DNA is severely restricted. In contrast to the results obtained in vivo, the biological activity of native and denatured transforming DNA is destroyed by BsuR in vitro, as is the transfecting activity of native and denatured SPP1 DNA. The sensitivity of denatured DNA, either with mixtures of the complementary strands or with separated single strands alone, is significantly lower than that of native DNA. The results are discussed in the context of possible mechanisms underlying the different responses of transforming and transfecting DNA to in vivo restriction by B. subtilis R cells.
研究了枯草芽孢杆菌R感受态细胞在体内的限制作用以及纯化的核酸内切酶BsuR在体外对天然DNA和变性DNA转化及转染的影响。结果表明，天然DNA或变性DNA的转化不受限制影响，而天然和变性SPP1 DNA的转染均受到严重限制。与体内获得的结果相反，天然和变性转化DNA的生物活性在体外被BsuR破坏，天然和变性SPP1 DNA的转染活性也是如此。无论是互补链混合物还是单独分离的单链，变性DNA的敏感性均显著低于天然DNA。结合枯草芽孢杆菌R细胞对转化和转染DNA体内限制的不同反应的潜在机制对结果进行了讨论。
