Takematsu H, Ohmoto Y, Tagami H
Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan.
Tohoku J Exp Med. 1990 Jul;161(3):159-69. doi: 10.1620/tjem.161.159.
Interleukin 1 (IL-1), which mediates a wide range of biological activities, is thought to play an important role in many inflammatory and immunologic diseases. Normal human epidermal keratinocytes constitutively produce IL-1. Based on our previous data indicating decreased IL-1 activity in psoriatic scale extracts, in the present study, we measured immunoreactive IL-1 alpha and beta levels in the suction blister fluids as well as in the psoriatic scale extracts using enzyme immunoassay for IL-1 alpha and beta. The results showed that although similarly low levels of IL-1 alpha were detectable in the suction blister fluid from normal and psoriatic lesional skin, and that no IL-1 beta was found in most of the blister fluids, indicating that IL-1 alpha is major IL-1 species produced by human skin. As compared to those in the blister fluids, IL-1 alpha levels in the horny tissue extracts were found to be much higher, and they were significantly higher in the orthokeratotic stratum corneum extracts than in the psoriatic scale extracts. However, gel filtration of the orthokeratotic horny tissue extracts demonstrated that constituents for immunoreactive IL-1 alpha and beta were quite variable depending upon the source of the horny tissues. The present study has confirmed that IL-1 levels in the psoriatic scale extracts are decreased when compared with those in the orthokeratotic horny tissue possibly due to an increased epidermal proliferation activity associated with its high turnover rate. The role of IL-1 psoriatic lesions remains unknown.
白细胞介素1（IL-1）介导多种生物学活性，被认为在许多炎症和免疫性疾病中起重要作用。正常人类表皮角质形成细胞可组成性产生IL-1。基于我们之前的数据表明银屑病鳞屑提取物中IL-1活性降低，在本研究中，我们使用IL-1α和β的酶免疫测定法测量了抽吸疱液以及银屑病鳞屑提取物中免疫反应性IL-1α和β的水平。结果显示，尽管在正常和银屑病皮损皮肤的抽吸疱液中可检测到相似低水平的IL-1α，且在大多数疱液中未发现IL-1β，这表明IL-1α是人皮肤产生的主要IL-1种类。与疱液中的相比，角质组织提取物中的IL-1α水平要高得多，并且在正角化角质层提取物中显著高于银屑病鳞屑提取物。然而，正角化角质组织提取物的凝胶过滤显示，免疫反应性IL-1α和β的成分因角质组织来源而异。本研究证实，与正角化角质组织相比，银屑病鳞屑提取物中的IL-1水平降低，这可能是由于与其高周转率相关的表皮增殖活性增加所致。IL-1在银屑病皮损中的作用仍不清楚。
