Takematsu H, Ohta H, Tagami H
Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan.
Arch Dermatol Res. 1989;281(6):398-400. doi: 10.1007/BF00455324.
Tumor necrosis factor (TNF)-alpha is a cytokine with multiple biological properties, particularly proinflammatory, apart from the induction of tumor necrosis. In order to elucidate the role of TNF-alpha in the pathogenesis of psoriasis, we have carried out bioassay and enzyme-linked immunosorbent assay for TNF-alpha in suction blister fluids and horny tissue extracts from psoriatic skin. Although bioassay showed some activities in the suction blister fluids and horny tissue extracts, there were no significant differences between the levels of activities from normal and psoriatic skin. They were at the background level and pretreatment of the samples with anti-TNF-alpha antiserum failed to abrogate the activities. ELISA confirmed the absence of TNF-alpha. Therefore, the present study could not verify that TNF-alpha plays a major role in the pathogenesis of psoriasis.
肿瘤坏死因子（TNF）-α是一种具有多种生物学特性的细胞因子，除了诱导肿瘤坏死外，尤其具有促炎特性。为了阐明TNF-α在银屑病发病机制中的作用，我们对银屑病皮肤的抽吸疱液和角质组织提取物中的TNF-α进行了生物测定和酶联免疫吸附测定。尽管生物测定显示抽吸疱液和角质组织提取物中有一些活性，但正常皮肤和银屑病皮肤的活性水平之间没有显著差异。它们处于背景水平，用抗TNF-α抗血清对样品进行预处理未能消除活性。酶联免疫吸附测定证实不存在TNF-α。因此，本研究无法证实TNF-α在银屑病发病机制中起主要作用。
