Verma R D, Sharma J K, Venkateswaran K S, Batra H V
Central Military Veterinary Laboratory, Meerut Cantt., India.
Vet Microbiol. 1990 Oct;25(1):77-85. doi: 10.1016/0378-1135(90)90095-d.
A dot enzyme-linked immunosorbent assay (dot ELISA) was developed for diagnosis of glanders in equines. The test was based on the detection of IgG antibodies to Pseudomonas mallei antigens bound to nitrocellulose coated on plastic strips (dipsticks), the reaction being amplified by an avidin-biotin system with biotinylated anti-horse IgG and horseradish peroxidase-avidin D. Sera from 810 normal, six naturally infected and 48 sensitized equines were tested by this assay, and results were compared with complement fixation, indirect haemagglutination and counter-immunoelectrophoresis tests. Dot ELISA had the highest sensitivity, and was superior to other tests in that it was rapid and easy to perform, the results were easy to interpret, the assay was not influenced by anti-complement activity, and it was able to detect antibodies at an early stage. Testing of serum at 1:200 dilution is proposed for epidemiological screening.
已开发出一种斑点酶联免疫吸附测定法（dot ELISA）用于诊断马鼻疽。该检测基于检测与包被在塑料条（试纸条）上的硝酸纤维素结合的鼻疽假单胞菌抗原的IgG抗体，反应通过生物素化抗马IgG和辣根过氧化物酶抗生物素蛋白D的抗生物素蛋白-生物素系统进行放大。用该测定法检测了810匹正常马、6匹自然感染马和48匹致敏马的血清，并将结果与补体结合试验、间接血凝试验和对流免疫电泳试验进行了比较。Dot ELISA具有最高的灵敏度，在快速、易于操作、结果易于解读、检测不受抗补体活性影响以及能够在早期检测到抗体方面优于其他检测方法。建议将血清以1:200稀释进行检测用于流行病学筛查。
