Niğde University, Department of Biology, Turkey.
Mutat Res. 2012 Jul 4;746(1):49-55. doi: 10.1016/j.mrgentox.2012.03.005. Epub 2012 Mar 28.
Contamination of plants with heavy metals could result in damage in DNA, such as mutations and cross-links with proteins. These altered DNA profiles may become visible in changes such as the appearance of a new band, or loss of an existing band, in the random amplified polymorphic DNA (RAPD) assay. In this study, various concentrations of copper and zinc salts were applied to cucumber seedlings during germination. Results displayed abnormalities in germination and also changes in root elongation, dry weight and total soluble protein level. All treatment concentrations (40, 80, 160, 240, 320, and 640mg/L) used in the study caused a decrease/delay in germination of the cucumbers to different extents. Inhibition or activation of root elongation was considered to be the first effect of metal toxicity in the tested plants. Application of the metal salts and the combined solutions on cucumber (Cucumis sativus L.) seedlings revealed similar consequences for total soluble protein level, dry weight and ultimately in inhibitory rates as well. The data obtained from RAPD band-profiles and genomic template stability (GTS) showed results that were consistent with the population parameters. In this regard, we conclude that molecular marker assays can be applied in combination with population parameters to measure genotoxic effects of heavy metals on plants.
重金属污染植物可能会导致 DNA 损伤，例如突变和与蛋白质的交联。这些改变的 DNA 图谱可能会在随机扩增多态性 DNA（RAPD）分析中表现为新带的出现或现有带的丢失等变化。在这项研究中，在黄瓜幼苗发芽期间施加了不同浓度的铜和锌盐。结果显示发芽异常，根伸长、干重和总可溶性蛋白水平也发生变化。研究中使用的所有处理浓度（40、80、160、240、320 和 640mg/L）都在不同程度上导致黄瓜发芽延迟或减少。根伸长的抑制或激活被认为是测试植物中金属毒性的第一效应。金属盐和混合溶液在黄瓜（Cucumis sativus L.）幼苗上的应用对总可溶性蛋白水平、干重以及最终的抑制率也产生了类似的后果。从 RAPD 带图谱和基因组模板稳定性（GTS）获得的数据与群体参数一致。在这方面，我们得出结论，分子标记分析可以与群体参数结合使用，以衡量重金属对植物的遗传毒性影响。
