Batir Muhammet Burak, Candan Feyza, Buyuk Ilker, Aras Sumer
Department of Biology, Faculty of Science and Letters, Celal Bayar University, Manisa, Turkey,
Environ Monit Assess. 2015 Apr;187(4):169. doi: 10.1007/s10661-015-4389-6. Epub 2015 Mar 8.
In this study, the effects of the heavy metal-polluted waters of the Gediz River, which flow into the Aegean Sea, and different concentrations of copper (Cu) solutions on maize (Zea mays L.) seedlings are investigated with physiological parameters and random amplified polymorphic DNA (RAPD) assay. Results displayed physiologically a significant difference in root and stem length between the control seedlings and the seedlings grown with the waters of the Gediz River. Also, the certain ascending concentrations of copper solution (80, 160, 320, 640, and 1280 ppm) caused a significant decrease in root and stem length of seedlings compared to the control seedlings. As a result of the waters of the Gediz River and copper solution treatment, the changes occurred in RAPD profiles of seedlings observed as variations like increment and/or loss of bands compared with the control seedlings. And these changes were reflected as a decrease in genomic template stability (GTS, changes in RAPD profile) derived by genotoxicity. RAPD band profiles and GTS values showed consistent results with physiological parameter. In conclusion, the study revealed the environmental risk and negative effect of waters of the Gediz River on maize seedlings and the suitability of RAPD assay for the detection of environmental toxicology.
在本研究中，通过生理参数和随机扩增多态性DNA（RAPD）分析，研究了流入爱琴海的格迪兹河重金属污染水以及不同浓度的铜（Cu）溶液对玉米（Zea mays L.）幼苗的影响。结果显示，对照幼苗与用格迪兹河水培养的幼苗在根长和茎长上存在生理上的显著差异。此外，与对照幼苗相比，一定浓度递增的铜溶液（80、160、320、640和1280 ppm）导致幼苗的根长和茎长显著降低。经过格迪兹河水和铜溶液处理后，与对照幼苗相比，幼苗的RAPD图谱出现了变化，表现为条带的增加和/或缺失等变异。这些变化反映为遗传毒性导致的基因组模板稳定性（GTS，RAPD图谱变化）降低。RAPD条带图谱和GTS值与生理参数显示出一致的结果。总之，该研究揭示了格迪兹河水对玉米幼苗的环境风险和负面影响，以及RAPD分析在检测环境毒理学方面的适用性。
