Laboratory of General and Clinical Parasitology, University of Paraná (UNIPAR), Praça Mascarenhas de Moraes s/n 87501-000, Umuarama, PR, Brazil.
Vet Parasitol. 2012 Aug 13;188(1-2):48-52. doi: 10.1016/j.vetpar.2012.03.011. Epub 2012 Mar 16.
A concomitant study was carried out, of the association of positive serology for Toxocara spp. in 90 children who played in public squares used for leisure, with the frequency with which each child used these areas, and the presence of eggs of Toxocara spp. in the sand or grass in these locations. The sand and grass of their peridomiciles and school playgrounds, as well as the feces of their dogs were also analyzed for Toxocara. Serum samples were tested for IgG antibodies to Toxocara canis excreted-secreted larval antigens by ELISA, and blood samples for eosinophilia. The water-sedimentation technique was used to evaluate the presence of parasite eggs in the sand and grass turfs, and in feces of the dogs that also frequented these locations. 16/90 (17.8%) of the children were seropositive for Toxocara spp. There was a positive association between seropositivity in children who played in the public squares six or seven times a week, with a parasite load above 1.1 eggs/g of sand, as well as with contamination of the peridomicile, even at less than 1.0 egg/g of sand. Eosinophilia, the habit of geophagy, age from one to four years, and the presence of parasitized pet dogs were also positively correlated with seropositivity in the children. Eggs were found in 15/15 (100%) of the public squares, 17/90 (18.9%) of the peridomiciles, 3/13 (23.1%) of the schools, and 12/41 (29.3%) of the dogs living in the peridomiciles investigated.
同时进行了一项研究，调查了 90 名在用于休闲的公共广场上玩耍的儿童中，Toxocara spp. 血清学阳性与每个儿童使用这些区域的频率以及这些区域的沙子或草地上是否存在 Toxocara spp. 卵之间的关联。还分析了这些地方的周边环境、学校操场的沙子和草以及他们的狗的粪便中的 Toxocara。通过 ELISA 检测血清 IgG 抗体来检测血清样本中 Toxocara canis 分泌/排泄的幼虫抗原，通过血液样本检测嗜酸性粒细胞。采用水沉淀技术评估沙草皮和狗粪便中寄生虫卵的存在情况，这些狗也经常光顾这些地方。90 名儿童中有 16/90（17.8%）血清阳性。在每周在公共广场上玩耍六到七次的儿童中，血清阳性与寄生虫负荷量超过 1.1 个卵/g 沙子以及周边环境受到污染呈正相关，即使沙子中的寄生虫负荷量低于 1.0 个卵/g 也是如此。嗜酸性粒细胞增多症、食土癖、1 至 4 岁年龄以及有寄生宠物狗也与儿童的血清阳性呈正相关。在 15/15（100%）个公共广场、17/90（18.9%）个周边环境、3/13（23.1%）个学校和 12/41（29.3%）个居住在周边环境中的狗中发现了卵。
