Department of Pharmacology, Osaka Dental University, Osaka, Japan.
Environ Health Prev Med. 2012 Nov;17(6):494-9. doi: 10.1007/s12199-012-0279-5. Epub 2012 Apr 6.
Salivary chromogranin A (CgA) levels and salivary flow rates were measured to evaluate the stress relief effect of laughter on the young and the elderly.
Thirty healthy volunteers (15 aged 20-25 years; 15 aged 62-83 years) performed a serial arithmetic task for 15 min and then watched a comedy video for 30 min. On a different day, as a control, they watched a non-humorous video after performing a task similar to the first one. Saliva samples were collected immediately before and after the arithmetic task, 30 min after completing the task (immediately after watching the film), and 30 min after watching the film (60 min after completing mental task). Salivary CgA levels were determined by enzyme-linked immunosorbent assay.
In the elderly group, salivary flow rates, which had declined by the end of the arithmetic task, were statistically significantly higher after watching the comedy video. In the young group, salivary CgA levels, which had increased by the end of the task, had statistically significantly declined after watching the comedy video. No such post-task changes were apparent in control results; in the young group, there was a statistically significant interprotocol difference in salivary CgA levels.
These findings suggest that laughter may relieve stress, particularly in the young people.
测量唾液嗜铬粒蛋白 A（CgA）水平和唾液流速，以评估笑声对年轻人和老年人的缓解压力的效果。
30 名健康志愿者（20-25 岁 15 名；62-83 岁 15 名）进行了 15 分钟的连续算术任务，然后观看了 30 分钟的喜剧视频。在不同的一天，作为对照，他们在完成类似的任务后观看非幽默视频。在算术任务之前和之后、任务完成后 30 分钟（看完电影后立即）以及观看电影后 30 分钟（完成心理任务后 60 分钟）收集唾液样本。通过酶联免疫吸附测定法测定唾液 CgA 水平。
在老年组中，在算术任务结束时下降的唾液流速在观看喜剧视频后统计学上显著增加。在年轻组中，在任务结束时增加的唾液 CgA 水平在观看喜剧视频后统计学上显著下降。对照结果没有出现这种任务后变化；在年轻组中，唾液 CgA 水平在协议之间存在统计学显著差异。
这些发现表明，笑声可能会缓解压力，特别是对年轻人而言。
