Arinbasarova Anna Yu, Medentsev Alexander G, Krupyanko Vladimir I
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Pr. Nauki 5, Russia.
Open Biochem J. 2012;6:11-5. doi: 10.2174/1874091X01206010011. Epub 2012 Mar 19.
This work studied the effect of gossypol on the mitochondrial respiratory chain of Yarrowia lipolytica. The compound was shown to inhibit mitochondrial electron transfer and stimulate generation of reactive oxygen species. The inhibition kinetics in oxidation of various substrates (NADH, succinate, α-glycerophosphate and pyruvate + malate) by isolated mitochondria was investigated. Analysis of the kinetic parameters showed gossypol to inhibit two fragments of the mitochondrial electron transfer chain: a) between coenzyme Q and cytochrome b with K(IIIi) of 118.3 μM (inhibition by the noncompetitive type), and b) at the level of exogenous NADH dehydrogenase with of K(Ii) 17.2 μM (inhibition by the mixed type).
这项工作研究了棉酚对解脂耶氏酵母线粒体呼吸链的影响。结果表明，该化合物可抑制线粒体电子传递并刺激活性氧的产生。研究了分离的线粒体对各种底物（NADH、琥珀酸、α-甘油磷酸和丙酮酸+苹果酸）氧化的抑制动力学。动力学参数分析表明，棉酚可抑制线粒体电子传递链的两个片段：a）辅酶Q和细胞色素b之间，K（IIIi）为118.3μM（非竞争性抑制类型）；b）在外源NADH脱氢酶水平，K（Ii）为17.2μM（混合型抑制）。
