Pekmezci Melike, Szpaderska Anna, Osipo Clodia, Erşahin Cağatay
Department of Pathology, Loyola University Medical Center, Maywood, IL 60153, USA.
Patholog Res Int. 2012;2012:947041. doi: 10.1155/2012/947041. Epub 2012 Mar 11.
Aims. To compare the results of estrogen and progesterone receptors (ER, PR), and human epidermal growth factor receptor-2 (HER2) expression status on biopsy and excision specimens and to evaluate the effect of cold ischemia time and/or formalin fixation on these biomarkers. Methods. Breast carcinomas that were diagnosed between 2007 and 2009 by core needle biopsy, and subsequently excised in our institution, were included in the study. Data regarding the tumor morphology, grade, and ER, PR, and HER2 status were retrospectively collected from the pathology reports. Results. Five out of 149 (3.4%) cases with ER-positive receptor status in the biopsy specimen became ER-negative in the subsequent excision specimen. Nine out of 126 (7.1%) cases with PR-positive receptor status in the biopsy specimen became PR-negative in the excision specimen. Receptor status change was predominantly seen in tumors with low ER and PR receptor expression. HER2 results were consistent between biopsy and excision specimens in all cases tested. Conclusions. Cold ischemia time and/or formalin fixation affect mainly ER and PR testing with low Allred scores and support the implementation of the ASCO/CAP guidelines. HER2 results, however, were not affected in our limited number of patients.
目的。比较活检和切除标本中雌激素受体（ER）、孕激素受体（PR）以及人表皮生长因子受体2（HER2）的表达结果，并评估冷缺血时间和/或福尔马林固定对这些生物标志物的影响。方法。纳入2007年至2009年间经粗针活检诊断并随后在本机构接受切除的乳腺癌病例。从病理报告中回顾性收集有关肿瘤形态、分级以及ER、PR和HER2状态的数据。结果。活检标本中ER受体状态为阳性的149例病例中有5例（3.4%）在随后的切除标本中变为ER阴性。活检标本中PR受体状态为阳性的126例病例中有9例（7.1%）在切除标本中变为PR阴性。受体状态改变主要见于ER和PR受体表达较低的肿瘤。在所有检测病例中，活检和切除标本的HER2结果一致。结论。冷缺血时间和/或福尔马林固定主要影响低Allred评分的ER和PR检测，并支持美国临床肿瘤学会/美国病理学家学会（ASCO/CAP）指南的实施。然而，在我们数量有限的患者中，HER2结果未受影响。
