Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, SP, Brazil.
Toxicol Mech Methods. 2012 Sep;22(7):497-501. doi: 10.3109/15376516.2012.680621.
The aim of the present study was to comparatively evaluate genomic damage and cellular death in exfoliated oral mucosa cells and peripheral blood from car painters. A total of 24 car painters and 19 healthy controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek mucosa (left and right side) mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the specific nuclear phenotypes. A total of 5 μL from peripheral blood was collected for the single cell gel (comet) assay. The results pointed out statistically significant differences (p < 0.05) of micronucleated oral mucosa cells from car painters. In addition, DNA damage was detected in peripheral blood cells by single cell gel (comet) assay. Nevertheless, exposure to car paints did not cause increases other nuclear alterations closely related to cytotoxicity such as karrhyorexis, pyknosis and karyolysis in buccal mucosa cells. In summary, the results of the present study suggest that car painters comprise a high risk group since paints can induce genotoxic and mutagenic effects in peripheral blood and oral mucosa cells, respectively.
本研究旨在比较评估汽车喷漆工脱落的口腔黏膜细胞和外周血中的基因组损伤和细胞死亡。在该环境中，共纳入 24 名汽车喷漆工和 19 名健康对照（未暴露个体）。个体的颊黏膜（左右侧）上皮细胞被机械刮除，置于固定液中，滴入干净的载玻片上，检查特定的核表型。从外周血中采集 5 μL 进行单细胞凝胶（彗星）分析。结果指出，汽车喷漆工的口腔黏膜有核微核细胞存在统计学显著差异（p < 0.05）。此外，单细胞凝胶（彗星）分析检测到外周血细胞的 DNA 损伤。然而，接触汽车涂料并未导致口腔黏膜细胞中与细胞毒性密切相关的其他核改变增加，如卡瑞奥雷西斯、固缩和核溶解。总之，本研究的结果表明，汽车喷漆工属于高风险群体，因为涂料可分别诱导外周血和口腔黏膜细胞的遗传毒性和致突变性。
