Martins Renato A, Gomes Guilherme A da Silva, Aguiar Odair, Ribeiro Daniel A
Department of Biosciences, Federal University of Sao Paulo, UNIFESP, 11060-001, Santos, SP, Brazil.
Environ Int. 2009 Oct;35(7):1062-5. doi: 10.1016/j.envint.2009.06.001. Epub 2009 Jun 25.
Owing to the influence of geno- and cytotoxicity on chemical carcinogenesis, studies have demonstrated that petroleum derivatives are able to induce genetic damage and cellular death with conflicting results so far. The aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from gas petrol attendants using two different anatomic buccal sites: cheek mucosa and lateral border of the tongue. A total of 23 gas petrol attendants and 23 health controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek and lateral border of the tongue mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the above nuclear phenotypes. The results pointed out significant statistical differences (p<0.05) of micronucleated oral mucosa cells from gas petrol attendants for both oral sites evaluated. In the same way, petroleum derivate exposure was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis, being the most pronunciated effects as those found in the lateral border of the tongue. No interaction was observed between smoking and petroleum exposure. In summary, these data indicate that gas petrol attendants comprise a high risk group for DNA damage and cellular death. It seems that the lateral border of the tongue is a more sensitive site to geno- and cytotoxic insult induced by petroleum derivates.
由于基因毒性和细胞毒性对化学致癌作用的影响，研究表明，石油衍生物能够诱导遗传损伤和细胞死亡，但迄今为止结果相互矛盾。本研究的目的是使用两个不同的口腔颊部解剖部位：颊黏膜和舌侧缘，比较评估汽油加油站工作人员脱落口腔黏膜细胞中的DNA损伤（微核）和细胞死亡（核固缩、核溶解和核碎裂）。本研究共纳入23名汽油加油站工作人员和23名健康对照者（未暴露个体）。对个体的颊部和舌侧缘上皮细胞进行机械性脱落，置于固定剂中，滴在干净载玻片上，检查上述核表型。结果指出，在评估的两个口腔部位，汽油加油站工作人员的微核化口腔黏膜细胞均存在显著统计学差异（p<0.05）。同样，石油衍生物暴露能够增加与细胞毒性密切相关的其他核改变，如核碎裂、核固缩和核溶解，其中在舌侧缘发现的影响最为明显。未观察到吸烟与石油暴露之间的相互作用。总之，这些数据表明，汽油加油站工作人员是DNA损伤和细胞死亡的高风险人群。似乎舌侧缘是对石油衍生物诱导的基因毒性和细胞毒性损伤更敏感的部位。
