Vahabi Surena, Amirizadeh N, Shokrgozar M A, Mofeed R, Mashhadi Abbas, Aghaloo M, Sharifi D, Jabbareh L
Dental School, Shahid Beheshti Medical University, Tehran, Iran.
Chang Gung Med J. 2012 Jan-Feb;35(1):28-37. doi: 10.4103/2319-4170.106169.
Recently, tissue engineering has been introduced as a regenerative treatment for bone defects. There is some evidence showing bone regeneration from mesenchymal stem cells (MSC) loaded on hydroxyapatite β-tricalcium phosphate (HA/TCP) as a scaffold in large defects. This study aimed to compare the quality and quantity of regenerated bone using Bio-Oss, HA/TCP and MSC loaded HA/TCP scaffolds.
Mesenchymal stem cells were aspirated from iliac crest bone marrow after extracting the first, second and third premolars and the first molar in five mature hybrid dogs. The cells were cultured and their osteogenic differentiation potential was evaluated after the third cell passage using Alizarin red staining in experimental conditions. The HA/TCP scaffold (3 × 3 × 3 mm) was loaded with undifferentiated mesenchymal stem cells. Bilateral bone defects were then prepared in the jaws using trephine burs. The defects were randomly filled with HA/TCP, Bio-Oss, or HA/TCP + MSCs. One defect served as a control and was left as an empty cavity. All defects except the control defect were covered with an absorbable membrane. Histological and histomorphometric evaluations were conducted after 6 weeks and data were subjected to analysis of variance (ANOVA) (p < 0.05).
The empty cavity demonstrated more bone formation (60.80%) than the HA/TCP (44.93%) and Bio-Oss (40.60%) (p < 0.05) groups. However, the difference from the HA/TCP + MSCs group was not significant (46.38%) (p > 0.05).
An MSC-loaded HA/TCP scaffold is a more effective alternative than Bio-OSS or HA/TCP in inducing bone regeneration.
近年来，组织工程学已被引入作为骨缺损的再生治疗方法。有证据表明，在大的骨缺损中，负载于羟基磷灰石β-磷酸三钙（HA/TCP）支架上的间充质干细胞（MSC）可实现骨再生。本研究旨在比较使用Bio-Oss、HA/TCP以及负载MSC的HA/TCP支架再生骨的质量和数量。
在拔除五只成年杂种犬的第一、第二和第三前磨牙以及第一磨牙后，从其髂嵴骨髓中抽取间充质干细胞。对细胞进行培养，并在第三次传代后，于实验条件下使用茜素红染色评估其成骨分化潜能。将未分化的间充质干细胞负载到HA/TCP支架（3×3×3毫米）上。然后使用环钻在颌骨上制备双侧骨缺损。缺损随机填充HA/TCP、Bio-Oss或HA/TCP+MSC。一个缺损作为对照，留作空腔。除对照缺损外，所有缺损均用可吸收膜覆盖。6周后进行组织学和组织形态计量学评估，并对数据进行方差分析（ANOVA）（p<0.05）。
空腔组的骨形成率（60.80%）高于HA/TCP组（44.93%）和Bio-Oss组（40.60%）（p<0.05）。然而，与HA/TCP+MSC组（46.38%）相比差异不显著（p>0.05）。
负载MSC的HA/TCP支架在诱导骨再生方面比Bio-OSS或HA/TCP更有效。
